Chip and method for real-time PCR (polymerase chain reaction) gene detection at polygenic mutation site

Abstract

The invention discloses a chip and method for real-time PCR (polymerase chain reaction) gene detection at a polygenic mutation site. The gene detection chip comprises a porous PCR plate, wherein a PCR reaction system is arranged in each pore of the porous PCR plate; the PCR reaction system comprises a plurality of primer pairs for the specific amplification of target gene targeting sequences, fluorescent probes, PCR buffer solutions, dNTPs (deoxyribonucleotide triphosphates) and a GoldTaq enzyme; each primer pair contains a continuous nucleotide sequence composed of at least 15 continuous nucleotides in target gene exons; and fluorescent molecules of the fluorescent probes emit fluorescent signals in the PCR amplification process. The detection rate of the method on big genes can be up to 98% or above.

Description

technical field [0001] The invention belongs to the technical field of real-time detection of multi-gene mutation sites, and in particular relates to a real-time PCR gene detection chip for multi-gene mutation sites and a detection method thereof. Background technique [0002] A DNA mutation is any detectable heritable change in the genetic material, but does not include genetic recombination. Mutations can be passed on to daughter cells and even passed on to offspring, resulting in mutant cells or individuals. In a multicellular organism, if a mutation occurs only in somatic cells, then the mutation is not passed on to offspring. This type of mutation is called a somatic mutation. But if the mutation occurs in the germ cells of the human body, then the mutation can be passed on to the next generation through gametes, and the same mutation will be produced in the somatic cells and germ cells of the offspring individual. This mutation is called a germline mutation. Since t...

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Problems solved by technology

[0026] The object of the present invention is to provide a real-time PCR gene detection chip and its detection method for multiple gene mutation sites, which solves the problem that in the prior art, multiple gene mutation sites of large genes, exon deletions of larger genes, Duplication and point mutation detection and other issues

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