Integrated horizontal gel electrophoresis device

Abstract

The invention discloses an integrated horizontal gel electrophoresis device. The device is mainly formed in the way that: an injection-molded horizontal electrophoresis device is provided with a gel tank between an anode tank and a cathode tank; the anode tank is separated from the gel tank by a first inner tank wall of the device; the inner tank wall is provided with a breakable weak part; the cathode tank is separated from the gel tank by another inner tank wall of the device; the inner tank wall is also provided with a breakable weak part; electrophoresis buffer solution is stored in the anode tank and the cathode tank; prefabricated gel is positioned in the gel tank, the bottom edge of the prefabricated gel is directly contacted with the inner bottom of an electrophoresis tank mould; and the gel tank, the anode tank and the cathode tank are combined together to constitute the ready-to-use integrated electrophoresis device. The invention provides the simple and easy-to-use improved electrophoresis device; the device has simple treatment process and operation steps, and structure and performance stability, and is time-saving and low in cost; and parts of components are disposable consumables, so that the time consumed for cleaning and maintaining equipment is saved.

Description

technical field [0001] The present invention is directed to gel electrophoresis systems, in particular to prefabricated horizontal monolithic gel electrophoresis systems and electrophoresis methods using the systems. Background technique [0002] As we all know, gel electrophoresis is used in research to separate and purify proteins, polypeptides, DNA, RNA, and various charged macromolecules, or as a subsequent analysis method for preparative steps. For example, gel electrophoresis is commonly used to separate and purify biological macromolecules for protein purity verification, protein identification, subsequent DNA sequencing, blotting experiments, mass spectrometry, polymerase chain reaction, restriction fragment length polymorphism analysis, cloning, or others Known techniques are used for further identification and other purposes. [0003] During electrophoresis, the gel is placed in a buffer-filled tank such that the top of the gel is just submerged in buffer. The ra...

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Problems solved by technology

[0006] But these traditional electrophoresis devices and electrophoresis methods are tedious and time-consuming because of the many steps required and the need to master the process of gel preparation, electrophoresis device assembly and performing electrophoresis

These electrophoresis methods also require a large amount of solution, usually more than 750 ml, and there may be leakage of the solution due to improper assembly of two plates to form an electrophoresis device

In addition, traditional electrophoresis systems are expensive and bulky, because they usually include buffer tanks, various clips, gel holders, solution compartment racks, electrophoresis tank covers, and various accessories required for gel preparation or electrophoresis

Traditional electrophoresis systems require cleaning and maintenance after electrophoresis is complete

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