Immunological measurement reagent for use in measurement of kl-6
An immunoassay, KL-6 technology, applied in the field of immunoassay reagents for KL-6 assay, can solve the problem that non-specific reaction samples cannot be sufficiently suppressed and the like
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[0064]
[0065] Briefly, an aqueous solution of 1100 g of distilled water, 200 g of styrene, 0.2 g of sodium styrene sulfonate, and 1.5 g of potassium persulfate dissolved in 50 g of distilled water was prepared in an Mix in a 2-L glass reaction vessel. The atmosphere of the vessel was replaced with nitrogen, and the mixture was polymerized at 70° C. for 48 hours with stirring.
[0066] After polymerization, the above solution was filtered through filter paper and latex particles were recovered. The diameter of the obtained latex particles was measured by image analysis of at least 100 particles. Photographs for latex particle image analysis were taken at 10,000× magnification using a transmission electron microscope (JEM-1010, JEOL). The average diameter is 0.2 μm.
Embodiment 1
[0069] (Preparation of solution containing latex particles immobilized with anti-KL-6 antibody (solution containing latex particles immobilized thereon with anti-KL-6 antibody; hereinafter referred to as reagent 2))
[0070] The anti-KL-6 antibody solution (5mM Tris-HCl, pH 8.0) adjusted to 0.7 mg / mL was added to an equivalent amount of a solution (5mM Tris-HCl, pH 8.0) containing 1.0% latex particles having an average particle size of 0.2 μm. 0). After stirring at 4°C for 2 hours, an equal amount of 2.0% BSA solution (5 mM Tris-HCl, pH 8.0) was added, and the mixture was stirred at 4°C for 1 hour. After centrifugation, the supernatant was discarded, and the pellet was resuspended in 5 mM Tris-HCl, pH 8.0. The mixture was diluted with 5 mM Tris-HCl, pH 8.0, so that the absorbance at a wavelength of 600 nm was 4.5 Abs. This solution was used as reagent 2.
[0071] Preparation of a solution containing a rheumatoid factor interference inhibitor (hereinafter, reagent 1).
[00...
Embodiment 2 to 4
[0080] Instead of 30 mM citrate buffer (pH 4.0) in Reagent 1 of Example 1, 30 mM citrate buffer at pH 4.5 (Example 2), pH 5.0 (Example 3) and pH 5.5 (Example 4) was used . Measurements were performed using the same reagents and the same assay as in Example 1.
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