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Preparation method of biological agent for preventing and treating ginseng diseases

A biological inoculum and disease technology, applied in the field of preparation of biological inoculants, achieves the effects of simple production operation, high spore formation rate and obvious effect

Active Publication Date: 2013-01-30
JILIN JIHAN BIO FERTILIZER
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Problems solved by technology

However, the research on the mechanism of ginseng disease control and field control effect of Bacillus subtilis biological agent is more obvious.

Method used

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  • Preparation method of biological agent for preventing and treating ginseng diseases
  • Preparation method of biological agent for preventing and treating ginseng diseases

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Experimental program
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Embodiment

[0016] The preparation method of the biological bacterial agent of preventing and treating ginseng disease, its steps are as follows:

[0017] 1. Strains: Bacillus subtilis.

[0018] 2. Bacteria activation culture:

[0019] (1) Incline cultivation

[0020] Culture medium preparation:

[0021] Divide the LB medium into test tubes, fill each tube with 10ml of medium, sterilize it by conventional methods, and make a slope. The Bacillus subtilis strains were inserted into the slant medium, placed in a constant temperature incubator at 37°C, and cultivated for 24 hours.

[0022] (2) Seed liquid preparation:

[0023] Divide the culture medium into 1000ml Erlenmeyer flasks, each bottle contains 200ml, sterilize by conventional methods, and transfer the slant-cultured strains into the Erlenmeyer flasks under aseptic conditions for shaking culture, the culture temperature is 37°C, the shaker speed is 200r / min, Incubate at constant temperature for 18 hours.

[0024] 3. Seed tank c...

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Abstract

The invention relates to a preparation method of a biological agent, namely the biological agent for preventing and treating ginseng diseases. The preparation method comprises the following steps: (1) performing strain culture; (2) preparing seed liquid; (3) performing culture in a seed tank; and (4) performing the culture in a fermentation tank. The using method is as follows: in the method for preventing and treating field diseases, the application concentration is 107cfu / ml, the application amount is 1.5L / m<2>, the application way adopts whole-plant uniform spraying and is matched with underground irrigation, the biological agent is prayed once every 10-20 days during the growth period of ginseng, and the biological agent needs to be sprayed three times at least during each growth period. The method is simple for production and operation, low in cost, complete in fermentation of thalli and high in spore formation rate. Tests prove that the bacillus subtilis biological agent has a certain bacteriostasis effect for the main diseases of the ginseng, such as ginseng phytophthora cactorum, ginseng rhizoctonia solani and ginseng alternaria panax.

Description

technical field [0001] The invention relates to a biological bacterial agent, namely a preparation method of the biological bacterial agent for preventing and treating ginseng diseases. Background technique [0002] In the prior art, ginseng belongs to Araliaceae, belongs to perennial herb, likes a cool and humid climate, and mostly grows in coniferous and broadleaf mixed forests or miscellaneous forests on gentle slopes or slopes of mountains with a small temperature difference between day and night at an altitude of 500-1100 meters. , is a precious Chinese herbal medicine. Nature and flavor are sweet, slightly bitter. It has the functions of warming, invigorating qi, promoting body fluid to calm the nerves, and replenishing qi. my country has a long history of artificially planting ginseng. The traditional ginseng planting is to adopt ecological imitation deforestation planting on the hillside, which has brought serious damage to the ecological environment and seriously ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/125
Inventor 李国君回云静吴长宝徐小明朱峰
Owner JILIN JIHAN BIO FERTILIZER
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