Sterile grafting method of peanut tissue culture seedlings

Abstract

The invention provides a sterile grafting method of peanut tissue culture seedlings. The method comprises the following steps of: taking sterilely-germinated peanut seedlings as parental stocks; cutting off cotyledons and parts above the cotyledons; longitudinally cutting the middle of lower plumular axes into notches; taking the peanut tissue culture seedlings as scions; cutting the lower end ofeach scion to form a V-shaped wound with the lengths of two inclined planes of 0.2-0.4 cm; inserting the scions into the middle parts of the notches to form a layer tight contact by the parental stocks and the scions; paving a grafting opening by using an opening sealing membrane to avoid losing water and accelerate healing; placing grafted seedlings into a solid culture medium to be sterilely cultured for 3-4 days; then transplanting the grafted seedlings into a sterilized seedling growing base material to be domesticated in a domestication room; then transplanting the seedlings into fields and managing the seedlings according to a field planting method. In the method provided by the invention, the parental stocks are grafted on the lower plumular axes with the notches below the cotyledons; the grafting operation is simple, the trouble of the need of usually removing lateral buds on the parental stocks is saved after the seedlings are transplanted to the fields, and the nutrition is sufficiently supplied to the scions; the tissue culture seedlings can be used for grafting when the lengths of the tissue culture seedlings are not more than 1 cm and the application range is wide; and the healing of the grafting wound can be accelerated and the dissemination of miscellaneous bacteria is prevented.

Description

technical field [0001] The invention relates to the field of peanut breeding, in particular to a method for aseptic grafting of peanut tissue culture seedlings. Background technique [0002] Peanut is an important oil crop and economic crop in my country, and plays an important role in agriculture and even the entire national economy. However, due to the narrow genetic basis and poor stress resistance of peanut cultivars, they are particularly susceptible to various pests and diseases, which seriously affect their yield and quality. Although breeders try to breed stress-resistant varieties, the selection of disease-resistant varieties has been a difficult problem due to the lack of resources of highly resistant varieties in peanut cultivars. With the development of biotechnology, the use of transgenic technology and in vitro mutagenesis technology to improve peanut cultivars has attracted extensive attention of researchers. The success of genetic transformation and in vitr...

AI Technical Summary

Problems solved by technology

The grafting incision is on the epicotyl, because there are two cotyledons, the grafting operation is troublesome; after transplanting in the field, the rootstock cotyledon axillary buds will grow the first pair of lateral buds, because there is a differentiated primordia, the lateral buds will continue to grow again after removal, Therefore, it is necessary to remove the side buds frequently, which increases the workload and also affects the growth and results of the scion.

[0004] And because the grafting otch of existing peanut grafting technology is epicotyl, and epicotyl is thinner, can't carry out grafting as scion for the regenerated seedling that stem is not elongated (because when seedling is small, internode is not elongated, relatively thick ), the seedlings need to grow to 2-3cm, and the internodes can be elongated before they can be used for grafting, which limits its application range