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Preparation method of hydrogel scaffold for amplifying human iPS (induced pluripotent stem) cells without differentiating

A technology for cultivating scaffolds and polymer hydrogels, which is applied in the direction of artificially induced pluripotent cells, animal cells, vertebrate cells, etc., can solve the problems of containing unknown proteins and complex components, and achieve safe and simple amplification and chemical structure. Clear, foreign body infection-free effect

Inactive Publication Date: 2012-03-21
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it is convenient to use, it not only has complex components and contains unknown proteins, but there is still a risk of cross-infection of different animals

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for preparing a hydrogel culture scaffold for undifferentiated expansion of human iPS cells, the steps are as follows:

[0019] Step 1: First prepare the electrolyte polymer monomer solution, that is, mix the electrolyte polymer monomer and the solvent in a mass ratio range of 1:50 to obtain the electrolyte polymer monomer solution, the solvent is a phosphate buffer solution of deionized water, and the electrolyte polymer monomer solution is high. Molecular monomers include negatively charged acrylic acid;

[0020] Step 2: Prepare a neutral polymer monomer solution, that is, mix the neutral polymer monomer and the solvent in a mass ratio range of 1:100 to obtain a neutral polymer monomer solution, and the solvent is a phosphate buffer solution with a pH value of 6.0 , the neutral macromolecular monomer includes hydroxyethyl methacrylate;

[0021] Step 3: Prepare polymer hydrogel, the prepared electrolyte polymer monomer solution and neutral polymer monomer sol...

Embodiment 2

[0027] A method for preparing a hydrogel culture scaffold for undifferentiated expansion of human iPS cells, the steps are as follows:

[0028] Step 1: First prepare the electrolyte polymer monomer solution, that is, mix the electrolyte polymer monomer and the solvent in a mass ratio range of 1:70 to obtain the electrolyte polymer monomer solution, the solvent is a phosphate buffer solution with a pH value of 7.0, and the electrolyte Polymers include methacrylic acid;

[0029] Step 2: Prepare a neutral polymer monomer solution, that is, mix the neutral polymer monomer and the solvent in a mass ratio range of 1:150 to obtain a neutral polymer monomer solution, and the solvent is deionized water or a pH value of 7.0 Phosphate buffered saline, neutral macromolecular monomers including methyl methacrylate;

[0030] Step 3: Prepare polymer hydrogel, the prepared electrolyte polymer monomer solution and neutral polymer monomer solution are in the range of 1:0.7 according to the mol...

Embodiment 3

[0036] A method for preparing a hydrogel culture scaffold for undifferentiated expansion of human iPS cells, the steps are as follows:

[0037] Step 1: First prepare the electrolyte polymer monomer solution, that is, mix the electrolyte polymer monomer and the solvent in a mass ratio range of 1:100 to obtain the electrolyte polymer monomer solution, the solvent is a phosphate buffer solution with a pH value of 8.0, and the electrolyte High molecular monomers include sodium styrene sulfonate;

[0038] Step 2: Prepare a neutral polymer monomer solution, that is, mix the neutral polymer monomer and the solvent in a mass ratio range of 1:200 to obtain a neutral polymer monomer solution, and the solvent is a phosphate buffer solution with a pH value of 8.0 , neutral macromolecular monomers include N-vinylpyrrolidone;

[0039] Step 3: Prepare polymer hydrogel, the prepared electrolyte polymer monomer solution and neutral polymer monomer solution are in the range of 1:1.0 according ...

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PUM

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Abstract

The invention provides a preparation method of a hydrogel scaffold for amplifying human iPS (induced pluripotent stem) cells without differentiating. The prepared hydrogel cell scaffold takes synthetic polymer hydrogel as a cell culture scaffold under conditions of not modifying any protein extracted from an animal body or relating to any trophocyte, and is an ideal biological material for safelyculturing human pluripotent stem cells with the advantages of definite chemical structure of synthetic polymer hydrogel, stable physicochemical performance, easiness in regulation and control, no foreign body infection, easiness in sterilization and low price. Therefore, a soft material culture system, which can safely, simply and conveniently amplify human iPS cells without differentiating, can be established.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a preparation method of a hydrogel culture scaffold for undifferentiated and expanded human iPS cells. Background technique [0002] Stem cells are the original source of cells for the human body and its various tissues. Its research has been paid great attention by governments and scientific circles all over the world, and has become one of the hot research fields of world science. The potential value of stem cell-based patent rights and know-how in the fields of life science, pharmacy, tissue engineering, basic medicine and medical application is immeasurable. [0003] Pluripotent stem cells (pluripotent stem cells) are a class of stem cells with both self-renewal and totipotency. Self-renewal refers to the ability of pluripotent stem cells to be cultured and passaged indefinitely in vitro, to amplify highly undifferentiated cells identical to themselves thro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/074C08F220/06C08F220/28C08F2/48C08F220/14C08F212/14C08F226/10
Inventor 陈咏梅刘健康刘振齐
Owner XI AN JIAOTONG UNIV
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