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Preparation method and application of effective part of solanaceae physalis plant

A technology of effective parts and Physalis genus, which is applied in the field of preparation of effective parts of Physalis plants of Solanaceae, can solve the problems of concentrated effective parts, inability to form, large elution range, etc., and achieves small dosage, low production cost and high quality. controllable effect

Active Publication Date: 2012-03-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the elution system adopted by the column chromatography is petroleum ether-acetone system, although the petroleum ether-acetone system is used to carry out column chromatography gradient elution can also achieve the effect of separating bitterin compounds in physalis, but this elution system, elution The desorption range is too large, and the effective part cannot be concentrated in the eluting solvent, so that a stable effective part cannot be formed, and there are technical defects

Method used

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  • Preparation method and application of effective part of solanaceae physalis plant
  • Preparation method and application of effective part of solanaceae physalis plant
  • Preparation method and application of effective part of solanaceae physalis plant

Examples

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Effect test

Embodiment 1

[0043] 1) Extraction: Dry the persistent calyx of the hanging golden lamp medicinal material at 60°C for 5 hours to obtain the dried persistent calyx of the medicinal material hanging golden lamp. Take 10Kg of the dried persistent calyx of the medicinal material hanging golden lamp, and add 6 times the weight of the medicinal material each time The aqueous ethanol solution (i.e. aqueous ethanol solution 60Kg) containing 95% by mass of ethanol was refluxed at 90°C for 3 hours for each extraction, and extracted 3 times in total (the total amount of aqueous ethanol solution was 180Kg), and the extracts were combined.

[0044] 2) Column chromatography: Concentrate the extract obtained in step 1) to near dryness under reduced pressure to obtain 837 g of thick extract, add 1 L of water to dissolve, then use 1 L of petroleum ether, 1 L of dichloromethane, and 1 L of ethyl acetate ester and 1L of n-butanol to obtain petroleum ether extract, dichloromethane extract, ethyl acetate extrac...

Embodiment 2

[0047] 1) Extraction: Dry the persistent calyx of the hanging golden lamp medicinal material at 60°C for 5 hours to obtain the dried persistent calyx of the medicinal material hanging golden lamp. Take 10Kg of the dried persistent calyx of the medicinal material of the hanging golden lamp, and add 8 times the weight of the medicinal material each time The aqueous ethanol solution containing 95% ethanol mass percent (i.e. 80Kg ethanol solution) was refluxed at 100°C for 2 hours for each extraction, a total of 2 extractions (a total of 160Kg ethanol solution), and the combined extracts.

[0048] 2) Column chromatography: Concentrate the extract obtained in step 1) to nearly dryness under reduced pressure to obtain 850 g of thick extract, add 1 L of water to dissolve, and then use 1 L of petroleum ether, 1 L of dichloromethane, and 1 L of ethyl acetate ester and 1L of n-butanol to obtain petroleum ether extract, dichloromethane extract, ethyl acetate extract, and n-butanol extract...

Embodiment 3

[0050] 1) Extraction: Dry the persistent calyx of the hanging golden lamp medicinal material at 60°C for 5 hours to obtain the dried persistent calyx of the medicinal material hanging golden lamp, take 10Kg of the dried persistent calyx of the medicinal material of the hanging golden lamp, and add 10 times the weight of the medicinal material each time The water (namely water 100Kg), reflux extraction at 100°C, each extraction time is 2h, a total of 2 extractions (total water 200Kg), combined extracts.

[0051]2) Column chromatography: Concentrate the extract obtained in step 1) to nearly dryness under reduced pressure to obtain 820 g of thick extract, add 1 L of water to dissolve, and then use 1 L of petroleum ether, 1 L of dichloromethane, and 1 L of ethyl acetate ester and 1L of n-butanol to obtain petroleum ether extract, dichloromethane extract, ethyl acetate extract, and n-butanol extract respectively, and the effective part of the hanging gold lamp is detected by thin la...

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Abstract

The invention discloses a method for preparing an effective part of a solanaceae physalis plant, which comprises the following steps of: 1) extracting a medicinal material by using an extraction solvent to obtain extract, wherein the medicinal material is at least one of fruit, rhizome and persistent calyx of the solanaceae physalis plant; and 2) concentrating the extract, adding water for dissolving, extracting by using extraction solvents with polarities from low to high to obtain extract, concentrating, loading to a normal phase column, and eluting by using an elution solvent; collecting eluent, and drying to obtain the effective part of the solanaceae physalis plant, wherein the extraction solvents at least comprise dichloromethane, and the elution solvent at least comprises dichloromethane and methanol in a volume ratio of (20-35):1. The preparation method is easy to operate, low in production cost and suitable for industrial production; and the effective part of the solanaceae physalis plant, which is prepared by the method, is high in physiological activity, has the advantages of controllable quality, low dosage and the like, is convenient to use, and can be used for preparing anti-tumor medicines.

Description

technical field [0001] The invention relates to the field of preparation and application of effective parts extracted from traditional Chinese medicines, in particular to a preparation method and application of effective parts of plants of the Physalis genus Solanaceae. Background technique [0002] There are about 120 species of plants in the genus Physalis of the Solanaceae family, most of which are distributed in tropical and temperate regions of America, and a few are distributed in Eurasia and Southeast Asia. There are 7 species in my country, 2 of which are variants, namely Physalis (P. .minima L.), P. angulata L., P. angulata L.var.illosa Bonati (variety), P. pubescens L., lantern fruit (P. peruxiana L.). The root, whole plant and fruit of Physalis plant in Solanaceae can be used as medicine. The ripe fruit can be eaten raw, with slightly sour taste, strong fragrance and bright fruit color. It is a fruit and vegetable rich in nutrients. food coloring agent. Solanace...

Claims

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Application Information

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IPC IPC(8): A61K36/81A61P35/00A61K131/00A61K125/00
Inventor 马忠俊季龙袁永雷
Owner ZHEJIANG UNIV
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