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Rapid propagation method for Photinia fraseri tissue culture

A tissue culture and heather technology, applied in the field of rapid propagation of red leaf heather tissue culture, can solve the problems of long rooting time and poor test repeatability, and achieve the effects of convenient material sampling, strong implementability and repeatability, and sufficient material sources.

Active Publication Date: 2013-03-27
SHANGHAI JIAOTONG UNIV
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Problems solved by technology

Ezequiel (2007) found that the addition of rhizosphere bacteria can significantly promote the development of roots and the growth of plants in the rooting research of Photinia fragrans tissue culture; previous studies mainly used conventional plant growth regulators 6-BA, NAA, KT, etc., the combination is different, the test repeatability is poor, and the rooting time is long

Method used

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  • Rapid propagation method for Photinia fraseri tissue culture
  • Rapid propagation method for Photinia fraseri tissue culture
  • Rapid propagation method for Photinia fraseri tissue culture

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Embodiment

[0027] The rapid propagation method of Photinia fragrans tissue culture of this embodiment includes the following steps:

[0028] Step 1: Take the stems of Photinia fragrans, trim them into explants, and induce culture after sterilization to obtain adventitious buds;

[0029] Take a two-year-old young and semi-ligninized stem of Photinia fragrans with 6 to 7 axillary buds (variety "Red Robin"), which is robust, disease-free and pest-free, trim off the leaves, and take the stem or top with axillary buds Rinse the surface dust with tap water, then soak in saturated washing powder solution for 2 minutes, then rinse with tap water, soak with 75% alcohol volume fraction on the ultra-clean workbench for 30 seconds, rinse with sterile water 3 times, and then Soak in a 0.1% mercury liter solution for 8 minutes, and then rinse with sterile water 5 times.

[0030] Cut the sterilized stem into small pieces with an axillary bud of about 1 cm, and then conduct induction culture. The differentia...

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Abstract

The invention relates to a rapid propagation method for Photinia fraseri tissue culture in the field of biological techniques, which comprises the following steps of: 1) taking a Photinia fraseri stem section, pruning the stem section into an explant and inducing after sterilization to obtain adventitious buds; 2) conducting subculture to the adventitious buds and thereafter conducting propagation culture; 3) conducting rooting culture to the adventitious buds after the propagation culture to obtain rooted seedlings; and 4) training the rooted seedlings and planting the rooted seedlings in sterilized culture mediums for culture to obtain regenerated Photinia fraseri plants. The rapid propagation method for the Photinia fraseri tissue culture has the following beneficial effects that since the Photinia fraseri stem section is used as the explant, is differentiated through adventitious bud induction and then is differentiated to obtain the regenerated plants, the materials can be obtained conveniently, the source of the materials is wide, the highest inductivity of the adventitious buds can reach 100 percent, the propagation speed is rapid, the propagation coefficient can reach 7.1, the rooting rate can reach more than 95 percent and the survival rate of transplanted seedlings can reach more than 80 percent.

Description

technical field [0001] The invention relates to a tissue culture method in the field of biotechnology, in particular to a rapid propagation method for tissue culture of Photinia fragrans. Background technique [0002] Photinia ( Photinia fraseri ) belongs to the genus Photinia (Rosaceae) Photinia ) is a hybrid of evergreen trees or shrubs, named for its bright red shoots and leaves. As a precious evergreen color-leaved tree species, Photinia fragrans has a wide range of uses, and it has good effects as hedges, green walls, modeling trees, and solitary plants. In addition, it has rich colors in four seasons, fast growth, strong germination, and pruning resistance. It can be used in multi-functional, multi-level, and three-dimensional applications according to garden needs. In foreign countries, especially in Europe, America and Japan, it is known as "the king of red leaves and hedges". [0003] Duan Zuan (2000) and Huang Xin (2004) used winter perennial shoots as explant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 曾丽龚霄雯汪晓沙丁国平张嫔薛芹芹王华君赵子刚
Owner SHANGHAI JIAOTONG UNIV
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