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Lactobacillus microcapsule and preparation method thereof

A lactic acid bacteria microcapsule and lactic acid bacteria technology, applied in the field of preservation of probiotics, can solve the problems of high production cost, high air inlet temperature, reduced product utilization rate and the like, and achieve the effects of low product cost, improved survival rate and strong stress resistance.

Active Publication Date: 2012-03-14
OCEAN UNIV OF CHINA +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Some research work has been carried out in this respect. For example, the Chinese invention patent with publication number CN1969889 (invention name: enteric multi-layer coated probiotic microcapsules and its preparation method) discloses a multi-layer coated probiotic microcapsule. The preparation method of the capsule, which uses lactic acid bacteria as the core material, uses the calcium alginate coating formed by the ion exchange between sodium alginate and calcium ions, and then uses the different properties of the isoelectric point of calcium alginate and chitosan to make chitosan Calcium alginate forms secondary coated microcapsules, which improves the stability of active lactic acid bacteria in the product to a certain extent, but its disadvantage is that the coating process is complicated, and the coating of multi-layer coating materials affects the release of lactic acid bacteria during use. , reducing the utilization of the product
Another Chinese invention patent with the publication number CN101063120 (a Gram-negative bacteria microcapsule and its preparation method) is to prepare the capsules by spray-drying the protective wall material and the culture medium bacteria liquid. Diameter is the microcapsule particle of 10~100 μm; But in this invention, protective wall material selects gum arabic, maltodextrin, β-cyclodextrin and carboxymethyl cellulose, and the price is more expensive, and production cost is high, is not suitable for industrialization use, and the air inlet temperature is high, which reduces the survival rate of bacteria to a certain extent

Method used

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  • Lactobacillus microcapsule and preparation method thereof
  • Lactobacillus microcapsule and preparation method thereof
  • Lactobacillus microcapsule and preparation method thereof

Examples

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Embodiment 1

[0017] The preparation of embodiment 1 lactobacillus plantarum microcapsules

[0018] 1. Concentrated Lactobacillus plantarum fermentation liquid

[0019] Centrifuge the fermentation culture solution of Lactobacillus plantarum at 4°C and 5000r / min for 10 minutes, wash the collected bacteria twice with normal saline, and resuspend the bacteria sludge to make a bacterial suspension for later use; the medium used can be selected MRS medium or any suitable medium for lactic acid bacteria.

[0020] 2. Preparation of spray mixture

[0021] Weigh 25g of soybean protein, 100g of skim milk, 25g of maltodextrin, 25g of glucose, and 2.5g of gelatin according to the mass ratio of 1:4:1:1:0.1, add water, stir and mix, and fully mix with the above bacterial suspension, Add water to adjust the mixture to 1000mL, in which the final concentration of bacteria is 2×10 9 cfu / mL, the weight-to-volume concentration of each component of the protective wall material is: soybean protein 2.5%, skim ...

Embodiment 2

[0024] Example 2 Preparation of Lactobacillus acidophilus microcapsules

[0025] 1. Concentrated Lactobacillus acidophilus fermentation broth

[0026] 4°C, 4000r / min, 10min, centrifuge the fermentation broth of Lactobacillus acidophilus, wash twice with normal saline, resuspend the sludge, and set aside;

[0027] 2. Preparation of spray mixture

[0028] Weigh 100g of soybean protein, 150g of skimmed milk, 75g of maltodextrin, 50g of glucose, and 25g of gelatin according to the mass ratio of 4:6:3:2:1, add water and mix well, and mix well with the above bacterial suspension, add water Adjust the mixture to 1000mL, in which the final concentration of bacteria is 1.0×10 10 cfu / mL, the weight-to-volume concentration of each component of the protective wall material is: soybean protein 10.0%, skim milk 15.0%, maltodextrin 7.5%, glucose 5.0%, and gelatin 2.5%.

[0029] 3. Spray drying treatment

[0030] A centrifugal spray dryer is used for spray drying treatment. The air inlet ...

Embodiment 3

[0031] The preparation of embodiment 3 Enterococcus faecium and Lactobacillus casei mixed bacteria microcapsules

[0032] 1. Concentrated fermented broth of Enterococcus faecium and Lactobacillus casei

[0033] 4°C, 4000r / min, 15min, centrifuge the fermentation broth of Enterococcus faecium and Lactobacillus casei respectively, wash twice with normal saline, resuspend the sludge, count, and divide Enterococcus faecium at a ratio of 1:1. The suspension is mixed with the Lactobacillus casei suspension, and set aside;

[0034] 2. Preparation of spray mixture

[0035] Weigh 50g of soybean protein, 100g of skimmed milk, 50g of maltodextrin, 25g of glucose, and 15g of gelatin according to the mass ratio of 2:4:2:1:0.6, add water, stir and mix, and suspend with the above-mentioned Enterococcus faecium and Lactobacillus casei. Mix the solution thoroughly, add water to adjust the mixture to 1000mL, and the final concentration of bacteria is 5.0×10 9 cfu / mL, the weight-to-volume conc...

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Abstract

The invention relates to a protective wall material for producing a lactobacillus microcapsule and the lactobacillus microcapsule prepared by using the protective wall material. In the lactobacillus microcapsule prepared by using the protective wall material, the viable bacteria rate reaches 98.9% which is far higher than that of the traditional like products; after the lactobacillus microcapsuleis stored for 6 months, the survival rate is still higher than 70%, so that the stability is high and the stress resistance is high. In addition, the protective wall material of the lactobacillus microcapsule comprises soybean protein, skimmed milk, malto dextrin, glucose and gelatin which are low in price, so that the price of products is lower than that of the traditional like products; when the lactobacillus microcapsule is prepared, a production process with lower spray drying air inlet and outlet temperature than the like products is utilized, and therefore, the survival rate of lactobacillus is easily increased and the energy consumption is reduced.

Description

technical field [0001] The invention belongs to the technical field of probiotic preservation, and in particular relates to a protective wall material for manufacturing lactic acid bacteria microcapsules and a preparation method thereof. The protective wall material can prolong the storage time of lactic acid bacteria and obtain a higher viable rate . technical background [0002] Lactic acid bacteria are one of the most widely used and effective probiotics in microbial additives. They are the main symbiotic bacteria in the digestive system of many animals and constitute a class of representative intestinal microorganisms. Among them, lactic acid bacteria represented by Lactobacillus and Bifidobacterium have been proven to be able to treat and prevent certain intestinal diseases of humans and animals clinically. At the same time, lactic acid bacteria are also one of the first two types of feed-grade biological additive strains that can be directly used in my country. Lacti...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K10/18
Inventor 彭红旎梁莉
Owner OCEAN UNIV OF CHINA
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