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Culture medium for growth of bovine-derived streptococcus agalactiae or streptococcus dysgalactiae

A technology of Streptococcus lactis and culture medium, which is applied in the field of medium for the growth of bovine Streptococcus agalactiae or Streptococcus dysgalactiae, and can solve the problem of unsuitable large-scale cultivation of Streptococcus dysgalactiae and Streptococcus agalactiae of cow mastitis To achieve the effect of increasing the concentration of bacterial antigens and immunogenicity, reducing production costs, and enhancing the immune effect of vaccines

Inactive Publication Date: 2012-02-15
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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Problems solved by technology

[0005] The test results showed that the growth cycle of Streptococcus agalactiae and Streptococcus dysgalactiae in ordinary nutrient broth was as long as 20-21 hours, and the number of bacteria at the peak growth period was only 6.0×10 8 -1.0×10 9 CFU, therefore, is not suitable for large-scale culture of Streptococcus dysgalactiae and Streptococcus agalactiae

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  • Culture medium for growth of bovine-derived streptococcus agalactiae or streptococcus dysgalactiae
  • Culture medium for growth of bovine-derived streptococcus agalactiae or streptococcus dysgalactiae

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[0019] The present invention will be explained below in conjunction with examples.

[0020] Preparation of Streptococcus agalactiae culture medium Weigh 17-23g tryptone, 2-5g yeast extract powder, 2-5g sodium chloride, 0.3-0.5g disodium hydrogen phosphate, 2-3g sodium bicarbonate, and combine the aforementioned groups Dissolve in 1000ml beef extract, adjust the pH to 7.7±0.1 with 2M sodium hydroxide solution, divide into Erlenmeyer flasks, and sterilize at 121℃ for 20 minutes. After the solution is allowed to cool, add whey 50 to the solution in a sterile manner. -60ml and 500g / L sterile glucose solution 20-25ml, shake well and set aside.

[0021] Preparation of Streptococcus lactis culture medium Weigh 17-23g tryptone, 2-5g yeast extract powder, 2-5g sodium chloride, 0.3-0.5g disodium hydrogen phosphate, 2-3g sodium bicarbonate, and combine the aforementioned groups Dissolve the aliquots in 1000ml beef extract, adjust the pH to 7.7±0.1 with 2M sodium hydroxide solution, divide in...

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Abstract

The invention discloses a culture medium for the growth of bovine-derived streptococcus agalactiae or streptococcus dysgalactiae. The components of the bovine-derived streptococcus agalactiae culture medium are as follows: 1000ml of beef extract, 17g to 23g of tryptone, 2g to 5g of yeast extract powder, 2g to 5g of sodium chloride, 0.3g to 0.5g of disodium hydrogen phosphate, 2g to 3g of sodium bicarbonate, 50ml to 60ml of whey and 20ml to 25ml of glucose solution by 500g / L. The components of the bovine-derived streptococcus dysgalactiae culture medium are as follows: 1000ml of beef extract, 17g to 23g of tryptone, 2g to 5g of yeast extract powder, 2g to 5g of sodium chloride, 0.3g to 0.5g of disodium hydrogen phosphate, 2g to 3g of sodium bicarbonate, 20ml to 25ml of calf serum, 50ml to 60ml of whey and 20ml to 25ml of glucose solution by 500g / L.

Description

Technical field [0001] The present invention relates to a bacterial culture medium, specifically a culture medium for the growth of bovine-derived Streptococcus agalactiae or Streptococcus degalactiae. This medium is mainly used for the large-scale cultivation of bovine-derived Streptococcus agalactiae or Streptococcus degalactiae, and the medium is used in the field of cow mastitis vaccine production. Background technique [0002] Cow mastitis has long been the most common and frequently-occurring disease among the three major diseases that affect the development of the dairy industry, causing huge economic losses to the dairy industry. According to Jones et al. (1984), about 70% of the loss caused by cow mastitis is caused by the decline in milk production caused by recessive mastitis. At present, there are about 220 million dairy cows in the world, and about one-third of them suffer from various types of mastitis. The annual loss due to mastitis is as high as 35 billion US do...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/46
Inventor 李宏胜李新圃罗金印陈炅然严作廷苗小楼王玲杨峰
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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