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Method for extracting purified total flavonoids from lindera reflexa hemsl by means of macroporous absorption resin

A technology of pore adsorption and total flavonoids is applied in the field of medicine to achieve the effect of simple method and development of medicinal value

Active Publication Date: 2012-02-15
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above situation, to overcome the defects of the prior art, the purpose of the present invention is to provide a method for extracting and purifying total flavonoids from the mountain cypress with macroporous adsorption resin, which can effectively solve the problem of extracting and purifying the total flavonoids from the mountain cypress, so as to meet the preparation and treatment of gastric ulcer. and analgesic and anti-inflammatory drugs

Method used

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  • Method for extracting purified total flavonoids from lindera reflexa hemsl by means of macroporous absorption resin
  • Method for extracting purified total flavonoids from lindera reflexa hemsl by means of macroporous absorption resin
  • Method for extracting purified total flavonoids from lindera reflexa hemsl by means of macroporous absorption resin

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Embodiment 1

[0013] In concrete implementation, the present invention is operated by the following steps:

[0014] 1. Preparation of the extract solution of Alpinia japonica: pulverize it into powder, then add 70% ethanol (or 70% ethanol, the same below) for ultrasonic extraction 3 times, extract 1 hour each time, 70% each time The amount of % ethanol added is 12 times of the weight of the sedge; combine the extracts three times, reclaim the ethanol under reduced pressure until the extract has no alcohol smell, and obtain a concentrated solution, which is diluted with water to be equivalent to 0.05mg (0.05mg) of crude drug per 1ml / ml crude drug) of the extract solution of the scorpion, for subsequent use;

[0015] 2. Macroporous adsorption resin treatment: the method is to first soak the macroporous adsorption resin with 95% ethanol for 12 hours, then wash it with 95% ethanol until the ethanol eluent mixed with water does not become white and turbid, and then wash it with distilled water ...

Embodiment 2

[0019] In specific implementation, the present invention can also be realized by the following steps:

[0020] 1. Preparation of the extract solution of Alpinia persicae: pulverize the persimmons persicae into powder, then add 80% ethanol (or 80% ethanol, the same below) for ultrasonic extraction 2 times, each extraction for 90 minutes, each time 80 The addition of % ethanol is 14 times of the weight of the sage; combine the extracts twice, reclaim ethanol under reduced pressure until the extract has no alcohol smell, and obtain a concentrated solution, which is diluted with water to be equivalent to 0.05mg (0.05 mg) of crude drug per 1ml. mg / ml crude drug), the extract solution of Lily of the mountain, for subsequent use;

[0021] 2. Macroporous adsorption resin treatment: the method is to first soak the macroporous adsorption resin with 95% ethanol for 12 hours, then wash it with 95% ethanol until the ethanol eluent mixed with water does not become white and turbid, and then...

Embodiment 3

[0025] The present invention can also be realized by the following steps in concrete implementation:

[0026] 1. Preparation of the extract solution of Saccharomyces esculenta: Grind Sinensis Sinensis into powder, then add 60% ethanol (or 60% ethanol, the same below) for ultrasonic extraction 4 times, the first two extractions are 60 minutes each time, The amount of ethanol added each time is 12 times the weight of the mountain cypress, and the last two extractions are 40 minutes each time, and the amount of ethanol added each time is 10 times the weight of the mountain cypress; the four extracts are combined, and the ethanol is recovered under reduced pressure to the extract No alcohol smell, get a concentrated solution, add water to dilute the concentrated solution to obtain a solution of the extract of the mountain perch containing 0.05 mg of crude drug (0.05 mg / ml crude drug) per 1 ml, and set aside;

[0027] 2. Macroporous adsorption resin treatment: the method is to firs...

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Abstract

The invention relates to a method for extracting purified total flavonoids from lindera reflexa hemsl by means of macroporous absorption resin, which can effectively solve the problem in extracting purified total flavonoids from lindera reflexa hemsl so as to prepare drugs for curing gastric ulcer, easing pain and resisting inflammation. The method is as follows: grinding the lindera reflexa hemsl to powder, adding ethanol and extracting through ultrasonication, recovering the ethanol under reduced pressure until the extract becomes non-alcoholic so as to get concentrated solution, and addingwater to dilute the concentrated solution to get lindera reflexa hemsl extractive solution; soaking macroporous absorption resin in ethanol, washing with ethanol, washing again with distilled water until the macroporous absorption resin becomes non-alcoholic, and then loading the macroporous absorption resin into a column; extracting the supernatant of the lindera reflexa hemsl extractive solution, after that, standing, washing with purified water to get rid of the impurities, eluting through ethanol, collecting the eluent, and finally recovering the ethanol under reduced pressure. In this way, purified total flavonoids from the active part of the root of the lindera reflexa hemsl can be obtained. The method provided by the invention is simple, stable and reliable, and the total flavonoids extracted from the lindera reflexa hemsl through the method can be effectively used in preparing the drugs for curing gastric ulcer, easing pain and resisting inflammation. The invention develops the medical value of the lindera reflexa hemsl and makes innovation in the field of medicine.

Description

technical field [0001] The invention relates to the field of medicines, in particular to a method for extracting and purifying total flavonoids from arborescens with macroporous adsorption resins. Background technique [0002] Lindera reflexa Hemsl. is a plant of the genus Lauraceae, which is a shrub or small tree. It is mainly distributed in Henan (Dabie Mountain, Tongbai Mountain and the southern part of Funiu Mountain), Jiangsu, Anhui and other places, and Xinyang area of ​​Henan Province is the most widely distributed and rich in yield, and is the main producing area of ​​Shanxi. The root or bark of the root or root bark is used as medicine. It is warm in nature and pungent in taste. It has the functions of promoting qi and relieving pain, invigorating the spleen and eliminating accumulation, promoting blood circulation and reducing swelling, etc., and the long-term clinical application of the root has achieved good results. It has been used to treat chronic gastritis a...

Claims

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Application Information

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IPC IPC(8): A61K36/54A61P1/04A61P29/00
Inventor 陈随清陈磊磊
Owner HENAN UNIV OF CHINESE MEDICINE
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