One-plate five-combined TORCH-IgM antibody colloidal gold rapid detection test strip and preparation method thereof

Abstract

The invention discloses a one-plate five-combined TORCH-IgM antibody colloidal gold rapid detection test strip. One end of a chromatographic membrane is connected with a gold-labeled pad, the other end of the gold-labeled pad is connected with a sample pad, and the other end of the chromatographic membrane is connected with an absorption pad; the absorption pad, the chromatographic membrane, the gold-labeled pad and the sample pad are arranged on a base plate; goat-anti-human IgM-labeled colloidal gold conjugate particles are adsorbed on the gold-labeled pad; detection lines of five TORCH antigens and a control line of a rabbit-anti-goat IgG antibody are coated on the chromatographic membrane; and the absorption pad is provided with labeling regions corresponding to the detection lines on the chromatographic membrane. The invention also discloses a preparation method of the one-plate five-combined TORCH-IgM antibody colloidal gold rapid detection test strip. The test strip can detect whether a pregnant woman suffers from the acute infection of the five TORCH pathogens through one-step blood serum sampling; and the result can be observed with the naked eye and can be easily accepted by the user. Thus, the test strip is especially suitable for basic health centers, and can effectively prevent congenital or perinatal newborn infection caused by the TORCH infection of the pregnant woman, thereby avoiding abortion, teratism, stillbirth, premature delivery, hypoplasia or the like.

Description

Technical field: [0001] The invention relates to the field of immunodiagnostic reagents, in particular to a one-plate five-unit TORCH-IgM antibody colloidal gold rapid detection test paper capable of simultaneously detecting five indicators of TORCH infection and a preparation method thereof. Background technique: [0002] my country is a country with a high incidence of birth defects. About 200,000 to 300,000 children with congenital deformities visible to the naked eye are born every year. In addition to defects that appear only months and years after birth, the total number of children with congenital defects is as high as 800,000 to 1.2 million each year, approximately Accounting for 4%-6% of the total number of births every year, a defective child will be born about every 30 seconds. [0003] In 1940, rubella was prevalent in Australia, and cataracts in newborns increased significantly in the following year. After investigation, the mother of the child had a history of ...

AI Technical Summary

Problems solved by technology

However, when using this test strip to detect serum samples containing two or more pathogen antibodies, after the colloidal gold-labeled antibody conjugate forms a complex with the first detection line, the remaining colloidal gold-labeled antibody conjugate reacts with the next detection line When the concentration drops, the detection sensitivity will decrease. When it flows to the third detection line for reaction, due to the lower concentration, the reaction detection sensitivity will be lower, and the specimen will appear false negative.

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