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Solution for sperm detection

A sperm and solution technology, applied in biological testing, material inspection products, etc., can solve problems such as ineffective promotion, large differences in physical and chemical properties of sperm nutrient solutions, large differences in penetration time and penetration distance, etc.

Inactive Publication Date: 2014-03-12
NAT RES INST FOR FAMILY PLANNING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although surrogates have some similarity to the function of women's cervical mucus, are inexpensive, and are readily available, most surrogates have significant flaws in trials
For example, native egg white is used as the penetrating medium, and the repeatability of the experiment is not good, and the penetrating time and penetrating distance are quite different from those of cervical mucus; the physical and chemical properties of sperm nutrient solution and cervical mucus are quite different. The penetration time and penetration distance are quite different from those of cervical mucus
The research results obtained by many types of cervical mucus substitutes are not recognized internationally and cannot be effectively promoted

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0019] The research of embodiment 1 sperm detection solution

[0020] 1. Test materials

[0021] Semen Collection: Semen was obtained from fertile donors who abstained for 2-5 days, resulting from masturbation. According to the WHO manual, semen was concentrated on flash slides according to the revised stringent standard (91±72×10 6 / ml, range 5.0-271), total motility (55±16%, range 6.0-81) and normal sperm morphology (20±7.8%, range 1.0-42).

[0022] Collection of Cervical Mucus: Cervical mucus is obtained from many different women who spontaneously ovulate after artificial insemination. All samples were collected at 3-day intervals before ovulation. Mucus is drawn from the cervical canal into a polyethylene tube. After collection, cervical mucus should be evaluated, including determination of mucus filamentous, fern-like crystals, viscosity, and pH (WHO, 2001). Normal cervical mucus (pH>7.0) was divided into vials and stored at 4°C for use within 7 days. Only normally ...

Embodiment 2

[0043] Example 2 Research on sperm detection solution for patients with oligospermia / asthenospermia

[0044] 1. Test materials

[0045] Semen collection: Semen is from oligo / weak sperm patients, produced by masturbation after 2-5 days of abstinence.

[0046] Collection of Cervical Mucus: Cervical mucus is obtained from many different women who spontaneously ovulate after artificial insemination. All samples were collected at 3-day intervals before ovulation.

[0047] Preparation of native egg whites: Egg whites are sucked from around the yolk layer of fresh eggs with a syringe.

[0048] Preparation of hyaluronic acid solution (solution B) containing L-carnitine: within 60 minutes before the test, dissolve L-carnitine in Tyrode buffer and mix with hyaluronic acid solution to form a solution containing L-carnitine hyaluronic acid solution. The concentration of L-carnitine is 10-20mM / L.

[0049] Control migration medium: Tyrode's solution containing BSA (Commonwealth Serum L...

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Abstract

The invention discloses a solution for sperm detection. The solution for sperm detection of the invention comprises hyaluronic acid and L-carnitine, and can be used for sperm-cervical mucus penetration tests. Thereinto, the hyaluronic acid is used as a substitute of human cervical mucus, and L-carnitine can protect sperm motility. Based on conditions that patients with oligozoospermia / asthenospermia have less sperm numbers and low sperm motility, the invention is further improved, and a sperm detection solution for patients with oligozoospermia / asthenospermia is prepared. The sperm detection solution for patients with oligozoospermia / asthenospermia has high sensitivity, and a high concentration of L-carnitine, and the L-carnitine can increase the oxidative damage resistance of sperms and protect sperm motility.

Description

technical field [0001] The invention relates to a sperm detection reagent. Specifically, it relates to a solution for sperm detection composed of hyaluronic acid and L-carnitine as main components. Background technique [0002] At present, the clinical evaluation of male fertility in my country mainly uses semen routine analysis (SRA) to understand the general characteristics of semen, sperm and seminal plasma. It includes sperm density, motility, motility and its morphological examination. Although SRA can provide information on spermatogenesis and its maturation, these conventional parameters are not reliable in predicting sperm fertilization ability [YUAN Yu-ying &.SHI Qi-xian: The Evaluation of sperm function. Reproduction &. Contraception18, 58, 1998.David G, Jouannet P, Martin-Boyce A. Spitz A & Schwartz D: Sperm counts in fertile and infertile men. Fertil Steril 31:453, 1979]. Sperm function evaluation is to detect the state of sperm performing a specific function....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50
Inventor 宁丽峰王慧萍于和鸣
Owner NAT RES INST FOR FAMILY PLANNING
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