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Combined extraction and purification technique for multiple active ingredients of polygonum multiflorum

A technology of combined extraction and active ingredients, applied in quinone separation/purification, organic chemistry, chemical instruments and methods, etc., can solve the problems of high cost, difficulty in large-scale preparation, low utilization rate of extraction solvent, etc., and achieve environmental friendliness and high purity Effect

Active Publication Date: 2012-01-18
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, in the reported extraction and purification methods, only a certain component in Polygonum multiflorum is extracted, and other components are ignored, and the utilization rate of the extraction solvent is low
However, the purification step adopts column separation technology, which is expensive in industrial production and is not easy to achieve large-scale production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Polygonum multiflorum was crushed into 70-mesh particles, 80% ethanol was added according to the ratio of 1g Polygonum multiflorum to 14mL, and extracted under reflux at 42°C with 1kW ultrasonic power for 2h. The extraction rates of stilbene glycosides, emodin and chrysophanol were 90.6%, 88.2% and 86.4%, respectively. The extract was filtered, the residue was washed once with ethanol, and the wash was combined into the extract. Concentrating the extract under reduced pressure at a temperature of 40°C into a thick paste and recovering the extraction solvent, the stilbene glycosides, emodin and chrysophanol can be concentrated into the thick paste without loss. Dissolve the thick paste with 8 times the volume of distilled water and filter, and the filter residue is set aside. The filtrate was concentrated under reduced pressure to 1 / 6 of the original volume, which was the crude stilbene glycoside. Add 10 mL of ether to the filter residue of the water extract to dissolv...

Embodiment 2

[0015] Polygonum multiflorum was crushed into 80 mesh particles, 80% ethanol was added according to the ratio of 1g Polygonum multiflorum to 12mL, and extracted under reflux at 40°C with 1kW ultrasonic power for 2.2h. The extraction rates of stilbene glycosides, emodin and chrysophanol were 91.2%, 88.3% and 85.7%, respectively. The extract was filtered, the residue was washed once with ethanol, and the wash was combined into the extract. Concentrating the extract under reduced pressure at a temperature of 45°C to a thick paste and recovering the extraction solvent, the stilbene glycosides, emodin and chrysophanol can be concentrated into the thick paste without loss. Dissolve the thick paste with 10 times the volume of distilled water and filter, and filter the residue for later use. The filtrate was concentrated under reduced pressure to 1 / 4 of the original volume, which was the crude stilbene glycoside. Add 8 mL of ether to the filter residue of the water extract to dissol...

Embodiment 3

[0017] Polygonum multiflorum was crushed into 80-mesh particles, 80% ethanol was added according to the ratio of 1g Polygonum multiflorum to 10mL, and reflux extraction was carried out at 38°C with 1kW ultrasonic power for 1.8h. The extraction rates of stilbene glycosides, emodin and chrysophanol were 88.3%, 86.7% and 84.3%, respectively. The extract was filtered, the residue was washed once with ethanol, and the wash was combined into the extract. Concentrating the extract under reduced pressure at a temperature of 50°C into a thick paste and recovering the extraction solvent, the stilbene glycosides, emodin and chrysophanol can be concentrated into the thick paste without loss. Dissolve the thick paste with 9 times the volume of distilled water and filter, and the filter residue is set aside. The filtrate was concentrated under reduced pressure to 1 / 5 of the original volume, which was the crude stilbene glycoside. Add 9 mL of ether to the filter residue of the water extrac...

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PUM

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Abstract

The invention relates to a combined extraction and purification technique for multiple active ingredients of a medicinal plant, in particular to a combined extraction and purification technique for multiple active ingredients of polygonum multiflorum. According to the method, stilbene glucoside, rheum emodin and chrysophanol in the polygonum multiflorum can be maximum extracted from the polygonum multiflorum into a solvent by using 70-80 percent of ethanol as an extraction solvent and are separated from starch in residual slag; water-soluble differences of the stilbene glucoside, the rheum emodin and the chrysophanol are used and water is used as a solvent so that the stilbene glucoside in alcohol extracts can be separated from the rheum emodin and the chrysophanol, and a crude product is obtained; the rheum emodin and the chrysophanol can be respectively separated from the alcohol extracts by successively using 5 percent of sodium carbonate and 5 percent of sodium hydroxide as solvents, and the rheum emodin and chrysophanol crude product can be obtained through an acid precipitation; and the polygonum multiflorum ethanol extract residual slag is washed by using water, and the starch with a purity of 85-87 percent can be obtained through precipitating water washing liquid. The invention has the characteristics of low cost, high efficiency, no pollution and high product purity.

Description

technical field [0001] The invention relates to a joint extraction and purification technology of various active components of medicinal plants, in particular to a joint extraction and purification technology of stilbene glycosides, emodin, chrysophanol and starch in Polygonum multiflorum multiflorum. technical background [0002] There are many reports on the single-component extraction and purification methods of different active components in Polygonum multiflorum, but there is no report on the comprehensive method of joint extraction, separation and purification of multiple active components in Polygonum multiflorum. In the reported extraction and purification methods of Polygonum multiflorum single component, the traditional extraction method is to use solvent leaching method or assisted ultrasonic method, after extraction, extract with ester or ether and concentrate into extract, and finally use column chromatography to extract the extract mixture. purification. In ad...

Claims

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Application Information

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IPC IPC(8): C07H15/203C07H1/08C07C50/34C07C46/10C08B30/00
Inventor 陈冠华吴娴石杰王坤
Owner JIANGSU UNIV
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