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Nisin extraction method

A technology of nisin and extraction method, which is applied in the direction of peptide preparation method, chemical equipment and method, and decapeptide. It can solve the problems of complex extraction process, high salt content, and product purity, and achieve a safe and simple method. Overcoming the effect of high salt content and low cost

Inactive Publication Date: 2012-01-04
LUOYANG QIHONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The object of the present invention is a new nisin extraction method specially developed for the defects of high salt content, complex extraction process and product purity in the above-mentioned prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In this embodiment, nisin is extracted according to the following steps:

[0023] (1) pH adjustment: Take 1 L of concentrated nisin fermented broth (prepared after bacterial cell adsorption, desorption, and filtration of bacterial cells, titer is 518,000 / ml), and adjust pH to 2.0 with hydrochloric acid.

[0024] (2) Precipitation: Add 1L of dichloromethane to the above concentrated nisin solution, stir thoroughly at 20°C, separate the upper aqueous phase after standing, mix the aqueous phase with the next batch of fermentation broth, and re-concentrate; the lower layer is separated from solid and liquid Reclaim dichloromethane to obtain nisin wet cake;

[0025] (3) Drying: Dry the nisin wet cake in a 50°C oven under reduced pressure under vacuum, and the dry cake is crushed to obtain food-grade nisin products.

[0026] The bioassay titer of the nisin product in this example is 20321 IU / mg, and the product purity is 50.80%.

Embodiment 2

[0028] (1) pH adjustment: take 1 L of concentrated nisin fermented broth (prepared by low-temperature vacuum concentration, titer 584,000), and adjust the pH to 1.7 with hydrochloric acid.

[0029] (2) Precipitation: Add 0.5L n-butanol to the above-mentioned nisin concentrated solution, stir thoroughly at 50°C, separate the lower aqueous phase after standing still, mix the aqueous phase with the next batch of fermentation broth, and re-concentrate; the upper solid-liquid Separating and recovering n-butanol to obtain nisin wet cake;

[0030] (3) Drying: Dry the nisin wet cake in a 60°C oven under reduced pressure under vacuum, and the dry cake is crushed to obtain food-grade nisin products.

[0031] The bioassay titer of the nisin product in this example is 26432 IU / mg, and the product purity is 66.08%.

Embodiment 3

[0033] (1) pH adjustment: take 1 L of concentrated nisin fermented broth (obtained by ultrafiltration after plate and frame filtration, titer 553,000), and adjust the pH to 3.0 with hydrochloric acid.

[0034] (2) Precipitation: add 0.2L carbon tetrachloride to the above nisin concentrated solution, stir thoroughly at 30°C, separate the upper aqueous phase after standing, mix the aqueous phase with the next batch of fermentation broth, and re-concentrate; the lower solid-liquid Separating and recovering carbon tetrachloride to obtain nisin wet cake;

[0035] (3) Drying: Dry the nisin wet cake in a 60°C oven under reduced pressure under vacuum, and the dry cake is crushed to obtain food-grade nisin products.

[0036] The bioassay titer of the nisin product in this example is 23081 IU / mg, and the product purity is 57.70%.

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Abstract

The invention relates to a nisin extraction method which is characterized by comprising the following steps: (1) adjusting the pH value of a nisin fermentation concentrated solution to 1.7-3.0, and mixing the nisin fermentation concentrated solution with a water-microsoluble organic solvent or water-insoluble organic solvent, wherein the organic solvent is any one of dichloromethane, n-butyl alcohol, ethyl acetate and carbon tetrachloride, or a combination of any two; and (2) stirring, standing, carrying out solid-phase separation to obtain a nisin wet cake, and drying while depressurizing invacuum to obtain the nisin product. The invention has the following main advantage: the nisin is precipitated by means of the organic solvent, thereby overcoming the defects of the existing process such as high salt content and low product purity, and ensuring that the product purity of nisin is higher than 50%. The method is safe, convenient, practical and low in cost.

Description

[0001] technical field [0002] The invention relates to a method for preparing a natural food preservative, in particular to a method for separating and extracting nisin from streptococcus lactis or a concentrated fermentation liquid of lactococcus lactis. [0003] Background technique [0004] Nisin, also known as lactococcus peptide or nisin, the English name Nisin, is a polypeptide with strong bactericidal effect produced by Streptococcus lactis or Lactococcus lactis in the metabolic process, primary structure It consists of 34 amino acids with a molecular weight of about 3510Da. Nisin is a natural food preservative that can inhibit most of the G + The growth and reproduction of bacteria and their spores has the advantages of strong antibacterial properties, non-toxicity to the human body, good water solubility, and good thermal stability. It has been widely used in the preservation and preservation of dairy products, canned food, meat products and beverages. [0005]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/315C07K1/14
Inventor 刘欣鲁吉珂
Owner LUOYANG QIHONG BIOTECH
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