A kind of method of extracting Budeliaoside A from Buddleja

A technology for assimilin A and drunken fish is applied in chemical instruments and methods, preparation of sugar derivatives, production of bulk chemicals, etc., and can solve the problems of few and unpublished methods for extracting assimilin A, and achieve extraction High efficiency, easy industrial production, simple operation effect

Inactive Publication Date: 2011-12-28
NANJING ZELANG MEDICAL TECH
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  • Abstract
  • Description
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Problems solved by technology

[0007] Through literature search, there are few public methods for extracting Buddlein A from Buddleja, especially the industrialized method has not yet been published.

Method used

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  • A kind of method of extracting Budeliaoside A from Buddleja

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Embodiment 1

[0016] The raw material of Buddleia was crushed to 60 mesh, 5kg was added to the supercritical extraction kettle, and liquid CO2 was introduced. The extraction pressure was set at 15MPa and the temperature was 40℃. After the above parameters were reached, the flow rate of liquid CO2 was adjusted to 20kg / h, and the dynamic extraction was carried out for 2 hours. Analyze the extract and discard it under the adjustment of pressure 6MPa and temperature 60℃, then adjust the pressure to 25MPa, and pass the entrainer 60% methanol solution at the same time, flow 3L / h, extract for two hours, analyze the extract, recover the reagent, and obtain the leaching The paste is dissolved in hot water and added to 300g polyamide resin for adsorption. First take 4BV 40% ethanol solution to elute impurities, then 3BV 90% ethanol solution to elute the target components, collect the target components under reduced pressure and concentrate, concentrate After crystallization, the crystalline material wa...

Embodiment 2

[0018] The raw material of Buddleia is crushed to 80 mesh, 5kg is added to the supercritical extraction kettle, liquid CO2 is introduced, the extraction pressure is 30MPa, the temperature is 50℃, after reaching the above parameters, the liquid CO2 flow rate is adjusted to 25kg / h, and the dynamic extraction is 2 hours Analyze the extract and discard it under the adjustment of pressure 8MPa and temperature 50℃, then adjust the pressure to 25MPa, and at the same time pass the entrainer 70% acetone solution, flow rate 2L / h, extract for two hours, analyze the extract, recover the reagent, and obtain the leaching Dissolve the paste in hot water, add it to 200g of polyamide resin for adsorption, first take 4BV of 40% ethanol solution to elute impurities, then use 3BV of 50% ethanol solution to elute the target components, collect the target components under reduced pressure and concentrate, concentrate Crystallize, filter out the crystals, recrystallize with 70% ethanol, and dry to obt...

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Abstract

The invention discloses a method for extracting Budiaoside A from Buddleja. The method steps are as follows: take Buddleia and grind it into 40-80 meshes, add it to a supercritical extraction kettle, perform gradient extraction with supercritical CO2 under the environment of extraction pressure 15-30MPa and temperature 40-50°C, analyze the extract, and collect the effective extract Reagent for material recovery, the resulting extract is dissolved in hot water, polyamide resin is added for adsorption, ethanol aqueous solution gradient elution, the active ingredients are collected and concentrated under reduced pressure, the concentrated solution crystallizes, the crystals are filtered out, dissolved in ethanol for recrystallization, and dried to obtain Buddleia Glycoside A. The method adopted in the invention has high extraction efficiency, low energy consumption, little pollution, simple process steps and is suitable for industrialized production.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to a method for extracting buddlein A from Buddleia. Background technique [0002] The principle of the supercritical CO2 fluid extraction (SFE) separation process is based on the relationship between the dissolution capacity of the supercritical fluid and its density, that is, the influence of pressure and temperature on the dissolution capacity of the supercritical fluid. In the supercritical state, the supercritical fluid is contacted with the substance to be separated to selectively extract the components of polarity, boiling point, and molecular weight in sequence. Because supercritical CO2 fluid extraction has the characteristics of high efficiency and no pollution, it is widely used in the production of natural products. [0003] Buddlenoid A is a flavonoid glycoside, CAS: 142750-32-1, molecular formula: C30H26O13, molecular structure: [0004] [0005] Buddleja A has the eff...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
CPCY02P20/54
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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