Method for culturing tissues of Buxus sempervives
A technology for tissue culture and golden-leaf boxwood is applied in the field of tissue culture of herbs to achieve the effects of increasing survival rate, increasing yield and increasing reproduction speed
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Embodiment 1
[0022] A method for tissue culture of Buxus aureus, the method comprising the following steps:
[0023] (1) Aseptic treatment
[0024] Pick the small buds from the upper part of Euonymus aureus, rinse them with tap water for 2 hours, put them on a clean bench, soak them in 75wt% ethanol for 30s, soak them in 1wt‰ mercury chloride solution for 15mins, and rinse them with sterile water for 5-6 times Afterwards, dry the surface water with sterile filter paper, cut the base of the buds into 1 cm lengths and inoculate them on the bud induction medium MS+6-BA2.0mg / L+NAA0.2mg / L;
[0025] (2) Differentiation and proliferation of buds
[0026] Three weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after one week. After culturing for one month, the callus with buds was cut off and placed in Proliferation medium MS+6-BA2.0mg / L+NAA0.2mg / L was cultu...
Embodiment 2
[0034] A method for tissue culture of Buxus aureus, the method comprising the following steps:
[0035] (1) Aseptic treatment
[0036] Pick the small buds from the upper part of Buxus aureus, rinse them with tap water for 2 hours, put them on a clean bench, soak them in 75wt% ethanol for 20s, soak them in 1wt‰ mercury chloride solution for 10mins, and rinse them with sterile water for 5-6 times Then use sterile filter paper to dry the surface water, cut the base of the buds into 1 cm lengths and inoculate them on the bud induction medium MS+6-BA1.0mg / L+NAA0.1mg / L;
[0037] (2) Differentiation and proliferation of buds
[0038] Three weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after one week. After culturing for one month, the callus with buds was cut off and placed in Proliferation medium MS+6-BA1.0mg / L+NAA0.1mg / L was cultured, and...
Embodiment 3
[0046] A method for tissue culture of Buxus aureus, the method comprising the following steps:
[0047] (1) Aseptic treatment
[0048] Pick the small buds from the upper part of Euonymus aureus, rinse them with tap water for 2 hours, put them on a clean bench, soak them in 75wt% ethanol for 40s, soak them in 1wt‰ mercury chloride solution for 20mins, and rinse them with sterile water for 5-6 times Then use sterile filter paper to blot the surface moisture, cut the base of the buds into 1 cm lengths and inoculate them on the bud induction medium MS+6-BA3.0mg / L+NAA0.3mg / L;
[0049] (2) Differentiation and proliferation of buds
[0050] Three weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after one week. After culturing for one month, the callus with buds was cut off and placed in Proliferation medium MS+6-BA3.0mg / L+NAA0.3mg / L was cultur...
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