Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Detection probe, detection kit and detection method for hepatitis B virus drug resistance gene mutation

A technology of hepatitis B virus and detection kit, which is applied in the field of molecular biology, can solve problems such as inability to eradicate the virus, and achieve the effects of avoiding uncertain factors, good specificity, and convenient operation

Inactive Publication Date: 2011-12-21
解码(上海)生物医药科技有限公司
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, nucleoside analogue drugs can only inhibit virus replication, but cannot eradicate the virus, so long-term use is required

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection probe, detection kit and detection method for hepatitis B virus drug resistance gene mutation
  • Detection probe, detection kit and detection method for hepatitis B virus drug resistance gene mutation
  • Detection probe, detection kit and detection method for hepatitis B virus drug resistance gene mutation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Detection of Hepatitis B Virus Drug Resistance Gene Mutation Sites

[0037]1. Take the serum of fasting subjects for DNA extraction

[0038] Collect 1ml of venous blood from fasting subjects, inject it into a sterile 1.5ml centrifuge tube, let stand for 2 hours at room temperature, transfer to 4 °C and let stand for 1 hour, centrifuge at 8000rpm / centrifuge for 5 minutes, and transfer 200μl of supernatant to another (If the measurement is performed within 5 days, the samples should be stored at 4°C. If the measurement time is delayed, the samples must be stored at -20°C. The samples should be stored below 4°C for transportation.)

[0039] Take 50 μl of the concentrated solution (main component: polyethylene glycol) into a certain number of 0.5 ml centrifuge tubes, and then add 50 μl of the serum sample obtained above, shake and mix, let stand at 4°C for 5 minutes, centrifuge at 15,000 rpm for 10 minutes, and discard. supernatant. Add 20 μl of lysis buffer (main compone...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for detecting mutation sites of multi-drug resistance genes of hepatitis B virus, a detection probe and a detection kit. The present invention can detect the mutation of the drug-resistant gene of hepatitis B virus very conveniently, and can detect the 18 mutant positions of the drug-resistant gene of hepatitis B virus currently disclosed by using one pair of PCR amplification primers and one detection probe any one or several of the points. Not only is the specificity good, but all mutation site detection can be completed in one detection process, which is convenient to operate, avoids many uncertain factors in multiple operations, and can greatly improve the detection accuracy. In addition, the detection method provided by the present invention can not only detect the mutation of the hepatitis B virus drug resistance gene, but also detect the genotyping of the hepatitis B virus in the same operation, with simple operation and high efficiency.

Description

technical field [0001] The invention belongs to the field of molecular biology, relates to medicine and biotechnology, and in particular relates to gene mutation detection related to the drug resistance of nucleoside analogs of hepatitis B virus. Background technique [0002] Hepatitis B virus (hepatitis B virus, HBV) belongs to the family Hepadnaviridae, and its genome is about 3.2Kb in length and is a partially double-stranded circular DNA. Hepatitis B is a widespread infectious disease worldwide, but the prevalence of HBV infection varies widely in different regions. According to the World Health Organization, about 2 billion people in the world have been infected with HBV, of which 350 million people are chronic HBV infection, and about 1 million people die of liver failure, cirrhosis and liver cancer caused by HBV infection every year. The 2006 National Hepatitis B Epidemiological Survey showed that the HBV surface antigen (HBsAg) carrier rate of the general population...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/70C12Q1/68C12N15/11
Inventor 潘加奎
Owner 解码(上海)生物医药科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com