Breeding method of new variety of banana
A banana and culture medium technology, applied in the field of tissue culture of herbaceous plants, to achieve the effect of increasing the reproduction speed, increasing the survival rate, and increasing the yield
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Embodiment 1
[0026] A method for cultivating a new banana variety, the method comprising the following steps:
[0027] (1) Aseptic treatment
[0028] Dig out the small buds at the base of the banana, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 30s, soak them in 1wt‰ mercury solution for 15mins, wash them with sterile water for 5-6 times, and use them Blot the surface moisture with sterile filter paper, cut the base of the buds into 1cm lengths and inoculate them on the bud induction medium MS+6-BA2.0mg / L+NAA0.2mg / L;
[0029] (2) Differentiation and proliferation of buds
[0030] Three weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after one week. After culturing for one month, the callus with buds was cut off and placed in Proliferation medium MS+6-BA1.0mg / L+NAA0.1mg / L was cultured, a...
Embodiment 2
[0038] A method for cultivating a new banana variety, the method comprising the following steps:
[0039] (1) Aseptic treatment
[0040] Dig out the small buds at the base of the banana, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 20s, soak them in 1wt‰ mercury chloride solution for 10mins, rinse them with sterile water for 5-6 times, and use them. Blot the surface moisture with sterile filter paper, cut the base of the buds into 1cm lengths and inoculate them on the bud induction medium MS+6-BA1.0mg / L+NAA0.1mg / L;
[0041] (2) Differentiation and proliferation of buds
[0042] Three weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after one week. After culturing for one month, the callus with buds was cut off and placed in Proliferation medium MS+6-BA2.0mg / L+NAA0.2mg / L was ...
Embodiment 3
[0050] A method for cultivating a new banana variety, the method comprising the following steps:
[0051] (1) Aseptic treatment
[0052] Dig out the small buds at the base of the banana, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in 75wt% ethanol for 40s, soak them in 1wt‰ mercury chloride solution for 20mins, rinse them with sterile water for 5-6 times, and use them. Blot the surface moisture with sterile filter paper, cut the base of the buds into 1cm lengths and inoculate them on the bud induction medium MS+6-BA3.0mg / L+NAA0.3mg / L;
[0053] (2) Differentiation and proliferation of buds
[0054]Three weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after one week. After culturing for one month, the callus with buds was cut off and placed in Proliferation medium MS+6-BA3.0mg / L+NAA0.3mg / L was c...
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