Preparation method of perfluorooctane sulfonate artificial complete antigen

A technology of perfluorooctane sulfonic acid, complete antigen, applied in chemical instruments and methods, animal/human proteins, instruments, etc.

Inactive Publication Date: 2011-12-14
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Preparation method of perfluorooctane sulfonate artificial complete antigen
  • Preparation method of perfluorooctane sulfonate artificial complete antigen

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Embodiment 1

[0006]

[0007] Dissolve perfluorooctane sulfonic acid 1 (10 mg) and compound 2 (14 mg) in DCM (3 ml), under nitrogen protection, add acidic resin to pH = 2, and react at room temperature for 10 hours; then centrifuge the above reaction mixture, take The supernatant was slowly added dropwise to a PBS buffer solution (20 ml) of BSA (40 mg). React at room temperature for 5 hours, then centrifuge, put the supernatant into a dialysis bag prepared by a dialysis membrane with a molecular weight of 10,000, suspend the dialysis bag in PBS buffer solution in a 2000ml beaker, dialyze at 4°C for 7 days, and then put the dialysis bag The liquid freeze-dried in is the complete antigen of synthetic perfluorooctane sulfonate. The molecular weight of the antigen determined by MALDI-TOF-MS mass spectrometry is: 70131.

Embodiment 2

[0009]

[0010] Compound 3 (100 mg), compound 5 (40 mg) were dissolved in MeOH:H 2 In O=1:1 (10ml), under the protection of nitrogen, the reaction was carried out at room temperature for 5 hours, conventional organic reaction treatment, and the pure compound 6 was obtained after column chromatography. Compound 6 (200 mg) and compound 7 (230 mg) were dissolved in pyridine, reacted at 0°C for 5 hours, processed by conventional organic reaction, and separated by column chromatography to obtain pure compound 8. Compound 8 (40 mg) was dissolved in DMF (2 ml), and then slowly added dropwise to a PBS buffer solution (20 ml) of BSA (140 mg). React at room temperature for 5 hours, then centrifuge, put the supernatant into a dialysis bag prepared by a dialysis membrane with a molecular weight of 10,000, suspend the dialysis bag in PBS buffer solution in a 2000ml beaker, dialyze at 4°C for 7 days, and then put the dialysis bag The liquid freeze-dried in is the synthetic complete anti...

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Abstract

The invention relates to a preparation method of artificial complete antigen of perfluorooctane sulfonates (PFOS), which belongs to the technical field of organic chemistry and immunology. Using the carboxylic acid group of perfluorooctane sulfonic acid, the perfluorooctane sulfonic acid is linked to the carrier protein directly or through a linking arm of appropriate length to prepare the complete antigen of perfluorooctane sulfonic acid. The carrier protein can be bovine serum albumin (BSA), ovalbumin (OVA), limpet hemocyanin (KLH) or human serum albumin (HSA). MALDI-TOF-MS mass spectrometry to determine the amount of PFOS on the carrier protein. The method is simple to operate, does not require special equipment and has low cost, and provides a basis for screening out its specific antibody and establishing an immunoassay method in the future.

Description

technical field [0001] The invention relates to a preparation method of perfluorooctane sulfonates (PFOS) artificial complete antigen, which belongs to the technical field of organic chemistry and immunology. Background technique [0002] Perfluorooctylsulfonic acid (PFOS) is an important fluorine-containing surfactant, which is mainly used in polymer additives, lubricants, fire extinguishing agents, insecticides, etc. Due to its chemical inertness and its extensive production and use, perfluorooctane sulfonic acid has caused serious environmental accumulation and pollution, and has become a new type of persistent organic pollutant that has attracted increasing attention after organochlorine pesticides and dioxins. Studies have shown that perfluorooctane sulfonic acid is a moderately toxic liver carcinogen, which can increase the risk of human cancer. In addition, perfluorooctane sulfonic acid has embryotoxicity and potential neurotoxicity, and is highly bioaccumulative. Va...

Claims

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Application Information

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IPC IPC(8): C07K14/765C07K14/77C07K14/795G01N27/62
Inventor 魏国华孙艳灵杜宇国
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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