A kind of co-cultivation method of freshwater rotifers and chlorella

Abstract

The invention discloses a method for separating dominant rotifers and co-culturing the dominant rotifer and chlorella vulgaris in an O3 / BAC (biological activated carbon) advanced treatment process, which comprises the following steps of: a. determining the rotifers in the O3 / BAC advanced treatment process: researching population structures of planktonic animals in various process flows of waterplant ozone activated carbon advanced wastewater treatment, sampling and respectively quantitatively and qualitatively analyzing; b. separating the dominant rotifers in the O3 / BAC advanced treatment process: acclimating in dark at room temperature over night, picking up health and lively adults by using a dropper for further culturing; c. culturing the chlorella vulgaris: grafting purified single chlorella vulgaris in a Blue-Green Medium culture solution; d. carrying out adaptability on the rotifers: directly grafting the rotifers with the initial grafting density of 10-30 / L in the cultured chlorella vulgaris solution; e. co-culturing: co-culturing according to the initial grafting density of the rotifers; and f. subcultring: if the rotifers in a culturing system is dead, transiting the rotifers for subcultring. Co-culturing the dominant rotifers indoors for a longer time in the O3 / BAC advanced treatment process is realized, and the density of the rotifers is maintained at 400-600 ind / ml. Particularly, experimental material is provided for safety control and risk assessment research of the rotifers in life drinking water.

Description

technical field [0001] The invention relates to the technical field of zooplankton cultivation, more specifically to a water plant O 3 The co-cultivation method of dominant rotifers and chlorella in BAC advanced treatment process is applicable to the co-cultivation of freshwater rotifers and chlorella. Background technique [0002] Rotifers generally do not cause pollution to water bodies, and they are an integral part of the organic environment of water bodies. Rotifers are the group with the highest reproduction rate and the strongest dispersal ability among multicellular animals, so they tend to become the dominant group of plankton in water bodies. Some rotifers are used as model organisms in the study of population ecology, molecular biology and ecotoxicology indoors, and some freshwater rotifers play an important role as good indicator organisms of water bodies and drinking water quality. [0003] Because rotifers reproduce in large numbers in the water body and roti...

AI Technical Summary

Problems solved by technology

And for O 3 The isolation and control of rotifers in the / BAC advanced treatment process is relatively rare

In addition, traditional culture methods are mostly used in actual aquaculture fisheries, which cannot meet the requirements of some experimental monophyletic purebreds, have a great impact on experimental results, and are even more unsuitable for O. 3 Study on the safety control of rotifers in / BAC process

Images