Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for extracting cantharidin with compound enzyme

A cantharidin and compound enzyme technology, which is applied in the fields of drug combination, sexual diseases, organic chemistry, etc., to achieve the effect of convenient follow-up processing, less product impurities, and increased white blood cells

Inactive Publication Date: 2011-12-07
BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
View PDF8 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The novel method of preparing cantharidin of the present invention has not yet been reported at home and abroad

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for extracting cantharidin with compound enzyme
  • A method for extracting cantharidin with compound enzyme
  • A method for extracting cantharidin with compound enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Take the southern big mylabris ( Mylabris phalerata Pallas ) 500g of medicinal materials, pulverized and passed through a 10-20 mesh sieve.

[0032] (2) Soak with water, add 0.8g of cellulase and 1.6g of pectinase per 500g of medicinal materials.

[0033] (3) Adjust the pH to 5.0 with HCl, enzymatically hydrolyze at 50°C for 2.5 hours, and then boil for 3 minutes to inactivate the enzyme.

[0034] (4) Extract the enzymatic hydrolysis product with chloroform-absolute ethanol-concentrated hydrochloric acid (volume ratio 1.5:1:1) in a 66°C water bath for 24 hours, filter the extraction product with hot suction, and add chloroform- Absolute ethanol-concentrated hydrochloric acid (volume ratio 1:2:1) leaching, hot suction filtration.

[0035] (5) The two filtrates were combined and evaporated to dryness, the obtained solid was dissolved in water-chloroform (volume ratio 2:1), and the lower organic phase was extracted.

[0036] (6) Rotate the lower organic phase to dry...

Embodiment 2

[0039] (1) Take 500g of Chinese mylabris medicinal material, crush it, and pass it through a 10-20 mesh sieve.

[0040] (2) Soak with water, add 1g of cellulase and 1.8g of pectinase per 500g of medicinal materials.

[0041] (3) Adjust the pH to 5.8 with HCl, enzymatically hydrolyze at 48°C for 3 hours, and then boil for 4 minutes to inactivate the enzyme.

[0042] (4) Extract the enzymatic hydrolysis product with chloroform-absolute ethanol-concentrated hydrochloric acid (volume ratio 1.5:1.5:1) in a water bath at 57°C for 23 hours, filter the extracted product with hot suction, and add chloroform- Absolute ethanol-concentrated hydrochloric acid (volume ratio 1:2:1.5) leaching, hot suction filtration.

[0043] (5) The two filtrates were combined and evaporated to dryness, the obtained solid was dissolved in water-chloroform (volume ratio 2:1.5), and the lower organic phase was extracted.

[0044] (6) Rotate the lower organic phase to dryness, add absolute ethanol-petroleum ...

Embodiment 3

[0047] (1) Take yellow and black mylabris ( M. cichorii Linnaeus ) 500g of medicinal materials, pulverized and passed through a 10-20 mesh sieve.

[0048] (2) Soak with water, add 1.5g of cellulase and 2.0g of pectinase per 500g of medicinal materials.

[0049] (3) Adjust the pH to 6.0 with HCl, enzymatically hydrolyze at 45°C for 2.5 hours, and then boil for 2 minutes to inactivate the enzyme.

[0050] (4) Extract the enzymatic hydrolysis product with chloroform-absolute ethanol-concentrated hydrochloric acid (volume ratio 1.5:1:1) in a 70°C water bath for 25 hours, filter the extracted product with hot suction, and add chloroform- Absolute ethanol-concentrated hydrochloric acid (volume ratio 1:2:1.5) leaching, hot suction filtration.

[0051] (5) The two filtrates were combined and evaporated to dryness, the obtained solid was dissolved in water-chloroform (volume ratio 2:1.5), and the lower organic phase was extracted.

[0052] (6) Rotate the lower organic phase to dryn...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for extracting cantharidin from compound enzyme. The method comprises the following steps of: performing enzymolysis on a mylabris medicinal material with cellulase and pectic enzyme; leaching a hydrolysate with a mixed liquid with the volume ratio of chloroform to absolute ethanol to concentrated hydrochloric acid of (1-2):(1-2):(1-2); performing suction filtration on a leached product in hot state and adding chloroform-absolute ethanol-concentrated hydrochloric acid in the volume ratio of (1-2):(1-2):(1-2) for leaching; combining filtrates obtained in two times and performing rotary steaming; dissolving the obtained solid by using a mixed liquid in the volume ratio of water to chloroform of (1-2):(1-2); extracting a lower layer organic phase and performing rotary steaming on the lower layer organic phase; adding a mixed liquor in the volume ratio of absolute ethanol to petroleum ether of (1-2):(1-2) into the obtained solid for dissolving; and recrystallizing with acetone to obtain cantharidin. Compared with the conventional extraction method, the method has the advantages of high extracting speed, low cost, time saving, labor saving, high extraction rate, lower impurity content, easiness for purification and the like, and is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy or raw material processing, and in particular relates to a method for extracting cantharidin by a compound enzyme method. Background technique [0002] Mylabris, including insects of the family Mylaridae, the southern mylabris (Perthus spp.) Mylabris phalerata Pallas or yellow and black mylabris (eye spot phlox) M. cichorii Linnaeus , is a kind of traditional Chinese medicinal material with a wide range of uses. Clinical and pharmacological studies have shown that in addition to anti-cancer, mylabris has various activities such as anti-virus, anti-fungal, aphrodisiac, and white blood cell increase. Cantharidin contains substances such as cantharidin, fat, wax, formic acid and pigment, among which cantharidin is the main active ingredient. As an anticancer drug with high application value, cantharidin has attracted more and more attention from the world. [0003] The extract...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/18A61P31/12A61P31/04A61P31/10A61P15/00
Inventor 葛喜珍杜晓霞田平芳林强王路
Owner BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com