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Breast cancer stem cell separation and purification method

A breast cancer stem cell, separation and purification technology, applied in the direction of tumor/cancer cells, animal cells, vertebrate cells, etc., can solve the problem of unseparated breast cancer stem cells, and achieve cost reduction, simple and rapid operation, and high sensitivity Effect

Inactive Publication Date: 2011-11-30
卢英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] There is no proprietary technology report on how to isolate specific breast cancer stem cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] Materials and sources used in the following examples

[0028] 1. Source of breast tumor tissue:

[0029] All cases were pathologically diagnosed with breast cancer and underwent radical mastectomy. All cases did not receive adjuvant therapy such as preoperative radiotherapy and chemotherapy, and postoperative pathological judgment of tumor stage and type, axillary lymph node metastasis. Immunohistochemistry was routinely performed on postoperative tumor samples to detect the expression of breast cancer-related receptors such as ER. The specimens sorted by MACS and flow cytometry were all taken from breast cancer lesions resected during operation, and the tissues near the edge of the tumor were excised under sterile conditions.

[0030] 2. Main reagents and instruments:

[0031] flow cytometer flow cytometer, FCM US FACSAnalysis

[0032] 45 micron pore size cell screen Changsha Boyou Biotechnology Co., Ltd.

[0033] Cell counting plate Changsha Boyou Biotechnology Co....

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PUM

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Abstract

The invention discloses a method for separating and purifying breast cancer stem cells using an immunomagnetic bead sorting system. The steps include: firstly preparing a single cell suspension, and secondly performing negative sorting by immunomagnetic beads to separate and remove normal cells in breast cancer cells , and finally perform immunomagnetic bead positive sorting to purify breast cancer stem cells. The method is simple and quick to operate, does not require expensive experimental equipment, has a high survival rate of cells after screening, and has high CD44++CD24- / + specificity, and will have broad application prospects in breast cancer stem cell research.

Description

technical field [0001] The invention relates to a method for separating and purifying cancer stem cells, in particular to a method for separating and purifying breast cancer stem cells from breast cancer tumor tissue by using immunomagnetic beads, and belongs to the field of biomedicine. Background technique [0002] Breast cancer is the most common malignancy in women. During its treatment, recurrence is an important factor affecting the curative effect, and the cause of recurrence is generally believed to be the residual breast cancer cells during surgical treatment, and the residual breast cancer cells that are resistant to chemotherapy and insensitive to radiotherapy, endocrine and other treatments. [0003] In recent years, the theory of cancer stem cells (CSC) has provided a new theoretical basis for breast cancer metastasis. This theory holds that only a small number of cells proliferate continuously in tumor tissue, while most tumor cells are in a state of non-proli...

Claims

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Application Information

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IPC IPC(8): C12N5/095
Inventor 卢英
Owner 卢英
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