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A preparation method of shogaol extract and its medical application

A technology of extract and dried ginger, which is applied in the field of preparation of 6-shogaol extract, can solve the problems of low yield, cumbersome steps, and increased cost, and achieve the effects of high yield, easy operation, and good quality of finished products

Inactive Publication Date: 2011-11-30
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has cumbersome steps, low yield and increased cost

Method used

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  • A preparation method of shogaol extract and its medical application
  • A preparation method of shogaol extract and its medical application

Examples

Experimental program
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Effect test

Embodiment 1

[0033]Accurately weigh about 7.5g of ginger supercritical fluid extract, fully mix it with 200ml of 60% ethanol acid aqueous solution (0.8M hydrochloric acid), heat and reflux at 100°C for 2 hours, after the reaction is complete, reclaim ethanol at 45°C, and obtain The acidic aqueous solution was extracted twice with 80ml ethyl acetate, the ethyl acetate solution was combined, the ethyl acetate was recovered, and concentrated to obtain about 7.5g of shogabe phenolic composition mainly composed of 6-shogaol, and 6-gingerol in the extract The content of enol was increased from 2.5% to 20% (quantitative by external standard method). Using 7.5g of the product after the reaction as raw material, 10g of 200-300 mesh column chromatography silica gel was used to mix the sample, and 150g of 200-300 mesh column chromatography silica gel was used for separation. The eluent petroleum ether and ethyl acetate were eluted in six gradients, respectively 50:1, 33:1, 25:1, 20:1, 17:1, 14:1 (vol...

Embodiment 2

[0035] Accurately weigh 1 g of dried ginger powder (purchased in Shandong), heat and reflux at 80° C. for 2 h with 40 ml of 90% ethanol, filter the resulting extract, recover ethanol, and concentrate to obtain about 99.2 mg of dried ginger extract. Accurately weigh 0.75g of dried ginger extract, fully mix it with 20ml of 80% ethanol acid aqueous solution (0.2M hydrochloric acid), heat and reflux at 100°C for 2 hours, after the reaction is complete, recover ethanol at 45°C, and use the obtained acid aqueous solution Extract twice with 8ml of ethyl acetate, combine the ethyl acetate liquid, reclaim ethyl acetate, concentrate to obtain about 0.75g of shogaol-based composition mainly composed of 6-shogaol, the content of 6-shogaol in the extract From the original 1.67% to 5.56% (external standard method quantification). Using 75g of the product after the reaction as raw material, mix the sample with 100g of 200-300 mesh column chromatography silica gel, and separate with 1500g of ...

Embodiment 3

[0037] Accurately weigh about 7.5g of ginger supercritical fluid extract, fully mix it with 200ml of 60% ethanol acid aqueous solution (0.4M hydrochloric acid), heat and reflux at 100°C for 2 hours, after the reaction is complete, reclaim ethanol at 45°C, and obtain The acidic aqueous solution was extracted twice with 80ml ethyl acetate, the ethyl acetate solution was combined, the ethyl acetate was recovered, and concentrated to obtain about 7.5g of shogabe phenolic composition mainly composed of 6-shogaol, and 6-gingerol in the extract The content of enol increased from 2.5% to 19% (quantitative by external standard method). Using 75g of the product after the reaction as raw material, mix the sample with 100g of 200-300 mesh column chromatography silica gel, and separate with 1500g of 200-300 mesh column chromatography silica gel. The eluent petroleum ether and ethyl acetate are eluted in six gradients, respectively : 1, 33: 1, 25: 1, 20: 1, 17: 1, 14: 1 (volume ratio), each...

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Abstract

The invention relates to the field of natural medicines, in particular to a preparation method of 6-shogaol extract and its medical application. Ginger supercritical fluid extract or dried ginger extract is extracted by heating reflux with ethanol-containing acid water, recovering ethanol, extracting the acid water solution with ethyl acetate, recovering ethyl acetate under reduced pressure to obtain a concentrate after extraction, and passing the concentrate through silica gel chromatography Column, gradient elution with petroleum ether and ethyl acetate, collecting the eluate and concentrating to obtain that, wherein the petroleum ether and ethyl acetate used for elution are eluted in six gradients, and the volume ratios are: 50:1, 33 :1, 25:1, 20:1, 17:1, 14:1, the 14:1 fractions were collected and concentrated. The preparation method of the present invention is easy to operate, the quality of the finished product is good, and the output is high. It only needs to be separated by silica gel column chromatography once after conversion to obtain the zingiberene phenolic composition, and the yield is very high, which can reach 60-65%, which is industrial production Bring great convenience.

Description

technical field [0001] The invention relates to the field of natural medicines, in particular to a preparation method of 6-shogaol extract and its medical application. Background technique [0002] Ginger is derived from the fresh or dried rhizome of Zingiber officinale Rosc. Ginger belongs to the traditional Chinese medicine of the same origin as medicine and food, and its clinical application is quite extensive, and it has a high degree of attention abroad. In recent years, there are abundant chemical and pharmacological reports on ginger and its main active chemical components. Studies have shown that the content of gingerols in ginger is very high, while shogaols are dehydrated products of gingerols, and their content is low. However, in terms of anti-inflammation, anti-oxidation and anti-tumor, the activity of shogaols is significantly better than that of gingerols [Comparative antioxidant and anti-inflammatory effects of [6]-gingerol, [8]-gingerol, [10]-gingerol and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C49/255C07C45/79A61P35/00
CPCY02P20/54
Inventor 齐炼文李萍马江
Owner CHINA PHARM UNIV
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