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Method for establishing fingerprint of Deng's herbal tea granules and standard fingerprint thereof

A technology of fingerprints and Denglao herbal tea, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that there are no literature reports on the quality control of Denglao herbal tea, and achieve simple methods, accurate authenticity, and reproducibility good effect

Inactive Publication Date: 2011-11-23
CHINA NAT ANALYTICAL CENT GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no literature report on the quality control of Denglao herbal tea in China

Method used

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  • Method for establishing fingerprint of Deng's herbal tea granules and standard fingerprint thereof
  • Method for establishing fingerprint of Deng's herbal tea granules and standard fingerprint thereof
  • Method for establishing fingerprint of Deng's herbal tea granules and standard fingerprint thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1. Detection of Fingerprint Spectrum of Deng Lao Herbal Tea Sugar-Free Granules

[0051] 1 Instruments and reagents

[0052] 1.1 Instruments Agilent 1100 high performance liquid chromatography system, ChemStation chromatography workstation.

[0053] 1.2 Reagents Chlorogenic acid, neochlorogenic acid, isochlorogenic acid A and isochlorogenic acid B reference substances; acetonitrile (chromatographically pure), methanol (chromatographically pure), twice redistilled water, phosphoric acid (analytical pure).

[0054] 2. High performance liquid chromatography

[0055] 2.1 Chromatographic conditions: the chromatographic column is filled with octadecylsilane bonded silica gel (Poroshell 120SB-C 18 Chromatographic column, 100×2.1mm, 2.7μm); mobile phase is gradient eluent composed of 0.5% phosphoric acid (A) and acetonitrile (B), column temperature: 25°C; UV detection: wavelength is 254nm; flow rate is 0.5ml / min; analysis time: 20min. Injection volume: 1 μl each of...

Embodiment 2

[0070] Example 2. Detection of standard fingerprints of 11 batches of Denglao herbal tea granules

[0071] Get 11 batches of Deng Lao herbal tea granules, detect according to the conditions of Example 1, and obtain the HPLC collection of samples of 11 batches of samples, as figure 1 shown. Through the comparison of 11 batches of HPLC chromatograms, the similarity evaluation is carried out, and the characteristic common peaks are determined:

[0072] There are 16 common peaks in the fingerprint spectrum. The retention time of the spectrum, the average value of the peak area and the total peak area are summarized as follows:

[0073] For peak No. 1, the average retention time RT is 2.26min, the RSD is 0.41%, and the peak area is 34.2495mAU*s, which is 4.00%, accounting for 3.22% of the total peak area.

[0074] For peak No. 2, the average retention time RT is 3.23min, the RSD is 0.60%, the peak area is 103.8625mAU*s, the RSD is 1.12%, accounting for 9.75% of the total peak are...

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Abstract

The invention discloses a method for establishing fingerprint of Deng's herbal tea granules and standard fingerprint thereof, and relates to a method for controlling quality of Chinese medicine preparation, in particular to a method for establishing HPLC (high performance liquid chromatography) standard fingerprint to detect quality of the Deng's herbal tea granules. The method comprises the following steps: (1) preparation of reference substance solution; (2) preparation of test substance solution; (3) chromatographic conditions: the chromatographic column takes octadecylsilane chemically bonded silica as filler; gradient elution is adopted, wherein the mobile phase of gradient eluent is 0.1-3%, and the gradient eluent consists of phosphoric acid and acetonitrile; the column temperature is 20-50 DEG C; the wavelength for ultraviolet detection is 240-262 nm; the flow velocity is 0.3-1.0 mL / min; and the time is 20-60 minutes; and (4) measurement: the fingerprint is obtained by HPLC. The method disclosed by the invention effectively represents the quality of Deng's herbal tea granules, is favorable for the monitoring of product quality, has the advantages of simplicity, stability, high precision and good reproducibility, and can quickly and accurately identify the true and false as well as the quality of the product.

Description

technical field [0001] The invention relates to a quality control method of Guangdong herbal tea, in particular to a construction method and a standard fingerprint of Denglao herbal tea granules by high performance liquid chromatography. Background technique [0002] Guangdong herbal tea is a kind of plant developed and summarized by the people of Lingnan according to the local climate and water and soil characteristics, guided by the theory of traditional Chinese medicine health preservation and based on Chinese herbal medicine in the process of long-term disease prevention and health care. drinks. Denglao herbal tea is one of the famous Guangdong herbal teas. It is composed of honeysuckle, chrysanthemum, coriander root, mulberry leaf, dandelion and licorice. Sore throat and other diseases. Now there are Deng Lao herbal tea sugar-containing granules, sugar-free granules, canned drinks, honey refining paste, herbal lozenges and other dosage forms on the market. [0003] A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 张中强杨运云邓洁薇宋化灿张翠仙李娜郭鹏然王冠华
Owner CHINA NAT ANALYTICAL CENT GUANGZHOU
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