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Alicyclobacillus chromogenic culture medium as well as application method and use thereof

A technology of chromogenic medium and bacillus, which is applied in biochemical equipment and methods, microbe determination/inspection, etc., and can solve the problems of weak growth support ability and poor specificity of the medium

Inactive Publication Date: 2013-07-24
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the defects of weak growth support ability and poor specificity of the culture medium currently used for detecting cycloaliphatic acid bacillus, the present invention provides a medium with strong growth support ability and high specificity for cycloaliphatic acid bacillus and its use method and use

Method used

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  • Alicyclobacillus chromogenic culture medium as well as application method and use thereof
  • Alicyclobacillus chromogenic culture medium as well as application method and use thereof
  • Alicyclobacillus chromogenic culture medium as well as application method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Example 1: Preparation of chromogenic medium for Bacillus cycloaliphatic acid of the present invention

[0088] Prepare four parts of A component (numbered as No. 1-4 respectively) according to the dosage data of each subcomponent of component A listed in Table 1 below, add deionized water to a final volume of 500mL, heat to dissolve, use 1NH 2 SO 4 Or adjust the pH value with 1N NaOH to the value listed in Table 2, then autoclave at 121°C for 15 minutes and take it out for later use.

[0089] Table 1: Amount of component A

[0090]

[0091] Prepare four parts of B component (numbered 1-4 respectively) according to the dosage data of each sub-component of component B in Table 2 below. Respectively add deionized water to a final volume of 500 mL, heat to dissolve, then autoclave at 121°C for 15 minutes and then take out for use.

[0092] Table 2: Dosage of component B

[0093]

[0094] Prepare the mother liquor of component C according to the data in Table 3 below, and code them ...

Embodiment 2

[0098] Example 2: Application temperature of the chromogenic medium of Bacillus cycloaliphatic acid of the present invention

[0099] 1. Preparation of the chromogenic medium of Bacillus cycloaliphatic acid of the present invention

[0100] Prepare the chromogenic medium for Bacillus cycloaliphatic acid as described in Example 1 above, and number it as No. 1-4. Take 6 pieces of each numbered chromogenic medium and divide them into 3 groups (2 pieces for each group) .

[0101] 2. Inoculation and culture

[0102] Select the representative strains of Bacillus cycloaliphatic acid in Table 4 below, use BAT broth to culture at 45°C for 48 hours, then use an inoculation loop to streak and inoculate each coloring medium, turn the plate over, The first group was placed in a 45°C incubator, the second group was placed in a 48°C incubator, and the third group was placed in a 50°C incubator. The colony morphology was observed after 48 hours.

[0103] Table 4: Growth status and colony characterist...

Embodiment 3

[0106] Example 3: Application time of the chromogenic medium of Bacillus cycloaliphatic acid of the present invention

[0107] 1. Preparation of the chromogenic medium of Bacillus cycloaliphatic acid of the present invention

[0108] Prepare the chromogenic medium for Bacillus cycloaliphatic acid as described in Example 1 above, and number it as No. 1-4. Take 6 pieces of each numbered chromogenic medium and divide them into 3 groups (2 pieces for each group) .

[0109] 2. Inoculation and culture

[0110] Select the representative strains of Bacillus cycloaliphatic acid in Table 4 below, use BAT broth at 45°C for 48 hours to increase the bacteria, then use an inoculating loop to streak and inoculate each coloring medium, turn the plate over, 50 Cultivation in an incubator at ℃, the first group was cultured for 24 hours, the second group was cultured for 48 hours, and the third group was cultured for 36 hours. Then observe the colony morphology.

[0111] Table 5: Growth status and colon...

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Abstract

The invention relates to a chromogenic culture medium for detecting alicyclobacillus as well as application method and use thereof. The chromogenic culture medium comprises a component A, a component B and a component C, wherein the component A comprises the following substances in parts by weight: 2-4 parts of a yeast extract, 5-10 parts of glucose, 0.25-0.4 part of CaCl2.2H2O, 0.3-0.5 part of MgSO4.7H2O, 0.1-0.3 part of (NH4)2SO4 and 2.5-3.5 parts of KH2PO4; the component B comprises the following substances in parts by weight: 15-20 parts of agar and 0.08-0.12 part of beta-galactosidase substrate; and 1 part by weight of the component C comprises the following substances in parts by weight: 0.18*10<-3>-0.2*10<-3> part of ZnSO4.7H2O, 0.16*10<-3>-0.18*10<-3> part of CuSO4.5H2O, 0.15*10<-3>-0.17*10<-3> part of MnSO4.H2O, 0.18*10<-3>-0.2*10<-3> part of COCl2.6H2O, 0.1*10<-3>-0.12*10<-3> part of H3BO3 and 0.3*10<-3>-0.5*10<-3> part of NaMoO4.2H2O. The chromogenic culture medium providedby the invention has strong capability of supporting growth of the alicyclobacillus and can be used for detecting the target alicyclobacillus with high specificity.

Description

Technical field [0001] The invention relates to a microbial chromogenic culture medium, in particular to a chromogenic culture medium for detecting Bacillus cycloaliphatic acid, and its use method and application. It belongs to the field of food hygiene microbiological safety inspection and monitoring, especially the detection of Bacillus cycloaliphatic acid in fruit and vegetable juice. Background technique [0002] Alicyclobacillus spp. (Alicyclobacillus spp.) is a group of heat-resistant, acidophilic and spore-forming bacilli that widely exist in nature. This type of bacteria grows well in an acidic environment (if in vegetable juice), and its spores can withstand acid The pasteurization process can still survive under the conditions. In 1971, Barland and Brock isolated Bacillus acidocaldarius in an acid-heat environment, which has a unique omega-cyclohexane fatty acid structure in its cell membrane, and this bacteria only works in acid-heat environments exist. In 1984, Cer...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04
Inventor 赵贵明陈颖赵勇胜杨海荣张琦劳华均
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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