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Alicyclobacillus chromogenic culture medium as well as application method and use thereof

A chromogenic medium and Bacillus technology, which is applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of weak growth support ability and poor specificity of the medium

Inactive Publication Date: 2011-11-23
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the defects of weak growth support ability and poor specificity of the culture medium currently used for detecting cycloaliphatic acid bacillus, the present invention provides a medium with strong growth support ability and high specificity for cycloaliphatic acid bacillus and its use method and use

Method used

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  • Alicyclobacillus chromogenic culture medium as well as application method and use thereof
  • Alicyclobacillus chromogenic culture medium as well as application method and use thereof
  • Alicyclobacillus chromogenic culture medium as well as application method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Embodiment 1: the preparation of cycloaliphatic acid bacillus chromogenic medium of the present invention

[0088] Prepare four parts of A component (respectively coded as No. 1-4) according to the amount data of each subcomponent of A component listed in Table 1, add deionized water to the final volume of 500mL, heat to dissolve, and use 1NH 2 SO 4 Or adjust the pH value to the value in Table 2 with 1N NaOH, then autoclave at 121°C for 15 minutes and take it out for use.

[0089] Table 1: Amount of component A

[0090]

[0091]Prepare four parts of B component (respectively coded as No. 1-4) according to the dosage data of each subcomponent of B component in Table 2. Add deionized water to a final volume of 500mL, heat to dissolve, and then autoclave at 121°C for 15 minutes before taking it out for use.

[0092] Table 2: B component dosage

[0093]

[0094] According to the data in Table 3, prepare the C component mother liquor, and code them into 4 numbers r...

Embodiment 2

[0098] Embodiment 2: the application temperature of cycloaliphatic bacillus chromogenic medium of the present invention

[0099] 1, prepare cycloaliphatic acid bacillus chromogenic medium of the present invention

[0100] According to above-mentioned embodiment 1, prepare cycloaliphatic bacillus chromogenic culture medium, and be compiled into No. 1-4, get each chromogenic medium of each numbering 6, be divided into 3 groups (each group 2 pieces) .

[0101] 2. Inoculation and cultivation

[0102] Select the representative strains of cycloaliphatic acid bacillus in the following table 4, use BAT broth to incubate at 45°C for 48 hours to enrich the bacteria, and then use an inoculation loop to take a ring and inoculate it on each color-developing medium, turn the plate over, and inoculate The first group was placed in a 45°C incubator, the second group was cultured in a 48°C incubator, and the third group was cultured in a 50°C incubator, and the colony morphology was observed...

Embodiment 3

[0106] Embodiment 3: the application time of cycloaliphatic bacillus chromogenic medium of the present invention

[0107] 1, prepare cycloaliphatic acid bacillus chromogenic medium of the present invention

[0108] According to above-mentioned embodiment 1, prepare cycloaliphatic bacillus chromogenic culture medium, and be compiled into No. 1-4, get each chromogenic medium of each numbering 6, be divided into 3 groups (each group 2 pieces) .

[0109] 2. Inoculation and cultivation

[0110] Select the representative strains of cycloaliphatic acid bacillus in the following table 4, use BAT broth to incubate at 45°C for 48 hours to enrich the bacteria, and then use an inoculation loop to take a loop and inoculate it on each color-developing medium, turn the plate over, 50 Cultivate in an incubator at ℃, cultivate the first group for 24 hours, the second group for 48 hours, and the third group for 36 hours, then observe the colony morphology.

[0111] Table 5: The growth status...

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Abstract

The invention relates to a chromogenic culture medium for detecting alicyclobacillus as well as application method and use thereof. The chromogenic culture medium comprises a component A, a component B and a component C, wherein the component A comprises the following substances in parts by weight: 2-4 parts of a yeast extract, 5-10 parts of glucose, 0.25-0.4 part of CaCl2.2H2O, 0.3-0.5 part of MgSO4.7H2O, 0.1-0.3 part of (NH4)2SO4 and 2.5-3.5 parts of KH2PO4; the component B comprises the following substances in parts by weight: 15-20 parts of agar and 0.08-0.12 part of beta-galactosidase substrate; and 1 part by weight of the component C comprises the following substances in parts by weight: 0.18*10<-3>-0.2*10<-3> part of ZnSO4.7H2O, 0.16*10<-3>-0.18*10<-3> part of CuSO4.5H2O, 0.15*10<-3>-0.17*10<-3> part of MnSO4.H2O, 0.18*10<-3>-0.2*10<-3> part of COCl2.6H2O, 0.1*10<-3>-0.12*10<-3> part of H3BO3 and 0.3*10<-3>-0.5*10<-3> part of NaMoO4.2H2O. The chromogenic culture medium providedby the invention has strong capability of supporting growth of the alicyclobacillus and can be used for detecting the target alicyclobacillus with high specificity.

Description

technical field [0001] The invention relates to a microbial chromogenic medium, in particular to a chromogenic medium for detecting cycloaliphatic acid bacillus, and its use method and purpose. It belongs to the field of food sanitation microbial safety inspection and monitoring, especially the detection of cycloaliphatic bacillus in fruit and vegetable juices. Background technique [0002] Alicyclobacillus spp. is a group of heat-resistant, acidophilic and spore-forming bacilli that widely exist in nature. This type of bacteria grows well in acidic environments (such as in vegetable juices), and its spores can withstand acidic conditions. conditions under which the pasteurization process can still survive. In 1971, Barland and Brock isolated Bacillus acidocaldarius in a hot and sour environment. The bacterial cell membrane has a unique ω-cyclohexane fatty acid structure, and this bacterium is only in a hot and sour environment. exist. In 1984, Cerny et al. also isolated ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
Inventor 赵贵明陈颖赵勇胜杨海荣张琦劳华均
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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