CD24 (glycosyl phosphatidyl inositol-linked surface mucin) and new use of CD24 antibody

A technology of CD24 and antibody, applied in the field of CD24 and CD24 antibody

Active Publication Date: 2011-11-23
PEKING UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the surface markers of human embryonic stem cell-derived pancreatic cells in the published research

Method used

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  • CD24 (glycosyl phosphatidyl inositol-linked surface mucin) and new use of CD24 antibody
  • CD24 (glycosyl phosphatidyl inositol-linked surface mucin) and new use of CD24 antibody
  • CD24 (glycosyl phosphatidyl inositol-linked surface mucin) and new use of CD24 antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1. Co-expression of CD24 with specific proteins in pancreatic precursor cells

[0062] 1. Determination of co-expression of CD24 and PDX1 in human embryonic stem cell-derived pancreatic cells

[0063] The specific steps of pancreatic differentiation culture are as follows:

[0064] 1): The human embryonic stem cell line H1 was replaced with cell culture solution I (seeding density was 80%), and cultured for 4 days at a temperature of 37°C and 5% carbon dioxide;

[0065] 2) The cells obtained in step 1) were directly replaced with cell culture medium II, and cultured for 6 days at a temperature of 37° C. and 5% carbon dioxide;

[0066] 3) The cells obtained in step 2) are directly replaced with cell culture medium III, and cultured for 5 days at a temperature of 37°C and 5% carbon dioxide to obtain pancreatic precursor cells;

[0067] The cell culture solution I is prepared according to the following method: DMEM / F12 medium, B27, activin A and wortmannin are mi...

Embodiment 2

[0103] Example 2. Enrichment of pancreatic precursor cells with anti-CD24 antibody

[0104] Human embryonic stem cell line H1 was purchased from WiCell Research Institute, NIH number WA01; human embryonic stem cell line H9 was purchased from WiCell Research Institute, NIH number WA09.

[0105] DMEM / F12 medium was purchased from Invitrogen, the catalog number is 11330-032; KnockOut SR medium was purchased from Invitrogen, the product catalog number was 10828-028; bFGF was purchased from PeproTech, the product catalog number was AF-100-18B;

[0106] B27 was purchased from Invitrogen, and the product catalog number was 17504044; activin A was purchased from PeproTech, and the product catalog number was 120-14; wortmannin was purchased from Sigma, and the product catalog number was W1628;

[0107] IMDM medium was purchased from Invitrogen, the catalog number was 12440-061; F12 medium was purchased from Invitrogen, the catalog number was 11765-054; RA was purchased from Sigma, the ...

Embodiment 3

[0138] Example 3. Verification of CD24 enrichment of differentiated pancreatic precursor cells on the human embryonic stem cell line H9

[0139] Method: basically the same as that described in Experiments 1 to 4, except that the human embryonic stem cell line H9 was used.

[0140] Results: There was no significant difference from the results described in Experiment 4.

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PUM

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Abstract

The invention discloses CD24 and a new use of a CD24 antibody. The new use refers to the use of the CD24 antibody in enrichment of precursor cells of pancreas. In the invention, the CD24 antibody can be used to enrich the precursor cells of pancreas to a certain degree, so that the effect of various signals from endothelium or interstitial substance on further differentiation maturity can be screened out accurately; and thus, the study on the whole pancreas differentiation process is promoted greatly.

Description

technical field [0001] The present invention relates to CD24 and the new application of CD24 antibody. Background technique [0002] Human embryonic stem cells can not only provide a sufficient source of donor islet cells for the future treatment of diabetes, especially type I diabetes, but also become an ideal in vitro research model for exploring the development of human pancreas. So far, some progress has been made in inducing the directed differentiation of human embryonic stem cells into pancreatic precursors and insulin-secreting cells. According to the step-by-step induction method, the differentiation process of human embryonic stem cells will simulate the development process of the pancreas in vivo. First, it will be induced to differentiate into intraembryonic endoderm, and then further induced to differentiate into pancreatic precursor cells and insulin-secreting cells. At present, the co-expression of some pancreatic development-related genes, such as PDX1, HNF1...

Claims

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Application Information

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IPC IPC(8): C12N5/071C12N5/0735
Inventor 邓宏魁蒋卫隋鑫张冬卉刘猛尹明时艳
Owner PEKING UNIV
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