Microbial agent, and its preparation method and application
A microbial inoculum, bioremediation technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as low degradation ability
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[0037] The present invention also provides a preparation method of the microbial agent, wherein the method comprises the preparation of Pseudomonas japonensis (Pseudoxanthomonas japonensis) with a preservation number of CGMCC No. Pseudomonas, Gordonia amicalis with preservation number CGMCC No.4794, Rhodococcus ruber with preservation number CGMCC No.4795 and alkanes with preservation number CGMCC No.4796 At least two species of Gordonia alkanivorans are inoculated in the culture medium for cultivation.
[0038] In a preferred embodiment, the preparation method includes: inoculating Pseudoxanthomonas japonensis (Pseudoxanthomonas japonensis) with a preservation number of CGMCC No. 4797 and at least one selected from the following strains in the culture medium : Pseudomonas (Pseudomonas) with preservation number CGMCC No.4793, Gordonia amicalis with preservation number CGMCC No.4794, Rhodococcus ruber with preservation number CGMCC No.4795 and Gordonia alkanivorans with a pres...
Embodiment 1
[0065] 1. Enrichment and isolation of petroleum hydrocarbon degrading bacterial strains
[0066] Weigh 5g of the oil-contaminated soil mixture and add it to a 250mL Erlenmeyer flask filled with 100mL enrichment medium, cultivate it at 28°C and 180r / min on a constant temperature shaker, and transfer 5mL of the culture medium to a fresh enrichment culture after 5 days base, cultured under the same conditions for 5 days, and continuously enriched and cultured for 25 days in this way, the culture solution was diluted serially and spread on solid PDA medium, and cultured at 28°C; after colonies grew on the plate, single colonies of different shapes were picked Separation and purification were carried out repeatedly by streak separation until a pure culture of a single strain was obtained, and the purified strain was preserved in the slant of beef extract peptone medium.
[0067] 2. Screening of strains with high degradation activity
[0068] Inoculate the above-mentioned purified ...
Embodiment 2
[0072] According to the experimental content and experimental methods recorded in "Common Bacteria Systematic Identification Manual" [4] and "Actinomycete Systematic Principles, Methods and Practice" [5], the preservation numbers obtained by screening are CGMCC No.4793, CGMCC The strains of No.4794, CGMCC No.4795, CGMCC No.4796 and CGMCC No.4797 were identified.
[0073] 1. Morphological analysis
[0074] The strains whose preservation numbers are CGMCC No.4793, CGMCC No.4794, CGMCC No.4795, CGMCC No.4796 and CGMCC No.4797 were analyzed for morphology and observed under an optical microscope. The results were as follows: Figure 1-16 shown.
[0075] The observation of the colony morphology was carried out on solid TSA medium. After the strains were streaked and inoculated, they were cultured at a constant temperature of 28°C for 24 hours to observe the colony morphology.
[0076] The colony of the strain with the preservation number CGMCC No.4793 is colorless and translucent...
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