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Preparation method of 2-keto-L-gulonic acid

A gulonic acid and ketone-based technology, which is applied in the field of preparation of 2-keto-L-gulonic acid, can solve the problems of reducing the reaction yield, and achieve the effects of facilitating acid production, reducing product inhibition, and increasing the conversion rate of sugar and acid

Inactive Publication Date: 2011-11-16
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, in the production process of domestic gulonic acid, feeding sodium carbonate is often used to control and adjust the pH. The product of this reaction is soluble sodium gulonic acid. In the later stage of the reaction, as the reaction progresses, the concentration of sodium gulonic acid gradually increases. It is easy to inhibit the formation of products in the fermentation reaction, thereby reducing the reaction yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 140m 3 The initial liquid volume in the fermenter is 30m 3 , the control initial sorbose concentration is 25mg / ml, and inoculum size is 30% (large bacterium and small bacterium mixed seed liquid), when residual sugar drops to 20mg / ml, begins to flow and add sorbose sugar solution, the concentration of sugar solution is 200mg / L, the fed-batch process controls the sugar content to 20-22mg / ml; at the same time, feeds calcium hydroxide to control the pH at 6.7-6.8 during the fermentation process.

[0025] During the fermentation process, the initial temperature was 29° C., the temperature rose to 30° C. at 30 hours, and 31° C. at 36 hours.

[0026] The fermentation period is 44 hours, and the final volume of fermentation is 100m 3 . After testing, the content of gulong acid in the fermentation broth is 105.1mg / ml, and the molar conversion rate is 94.5%.

Embodiment 2

[0028] 140m 3 The initial liquid volume in the fermenter is 35m 3 , the control initial sorbose concentration is 25mg / ml, and inoculum size is 30% (big bacterium and small bacterium mixed seed liquid), when residual sugar drops to 20mg / ml, begin to flow sorbose sugar liquid, the concentration of sugar liquid is 200mg / ml L, the sorbose content is controlled at 20-22 mg / ml during the feeding process; the pH is controlled at 6.8-7.0 by feeding calcium hydroxide during the fermentation process.

[0029] During the fermentation process, the initial temperature was 29 °C, the temperature rose to 30 °C in 24 hours, and the temperature rose to 31 °C in 30 hours.

[0030] The fermentation period is 46 hours, and the final volume of fermentation is 106m 3 . After testing, the content of gulong acid in the fermentation broth is 106 mg / ml, and the molar conversion rate is 94.79%.

Embodiment 3

[0032] 140m 3 The initial liquid volume in the fermenter is 35m 3 , initial sorbose concentration is 23mg / ml, inoculum size is 30% (big bacterium and small bacterium mixed seed liquid), when residual sugar drops to 20mg / ml, begins to flow and add sorbose sugar liquid, the concentration of sugar liquid is 200mg / ml. L, the sorbose sugar content is controlled at 20-22 mg / ml during the feeding process; the pH is controlled at 6.7-6.8 by feeding calcium hydroxide during the fermentation process.

[0033] The initial fermentation temperature is 29°C, the temperature rises to 30°C in 30 hours, the temperature rises to 31°C in 36 hours, the fermentation cycle is 48 hours, and the final volume of fermentation is 108m 3 .

[0034] After testing, the content of gulong acid in the fermentation broth is 107 mg / ml, and the molar conversion rate is 95.4%.

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Abstract

The invention relates to a preparation method of 2-keto-L-gulonic acid. In the invention, since a pH value in the fermentation process is controlled by utilizing calcium hydroxide instead of sodium carbonate, inhibition of acid production by a high-concentration product can be avoided, fermentation reaction progress is facilitated, the content of the gulonic acid at the fermentation destination reaches 105-110 mg / ml, the saccharic acid conversion rate is 94-96%, the concentration of produced gulonic acid is improved, the technical difficulty can be reduced, the operation is simple, the after extraction is easy, and the equipment utilization rate is improved.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a preparation method of 2-keto-L-gulonic acid. Background technique [0002] At present, domestic production of gulonic acid mostly adopts a two-step fermentation method, and the two-step fermentation method usually uses a mixed strain of bacteria for fermentation. The two most commonly used strains of this mixed strain are Gluconobacter oxidans (commonly known as small bacteria), and the other The species is Bacillus megaterium (commonly known as large bacteria), in which the small bacteria are acid-producing bacteria, and the intracellular secretion of L-sorbose dehydrogenase can convert sorbose into 2-keto-L-gulonic acid, but the growth is slow when cultured alone. The ability to produce acid is low; large bacteria cannot produce acid, but they can serve as companion bacteria to small bacteria, play an auxiliary role in fermentation, and promote the gr...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P7/60C12R1/01C12R1/11
Inventor 李荣杰穆晓玲符秀迪李维理李海燕
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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