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Method for repairing microorganisms in heavy metal contaminated soil

A technology for microbial remediation and contaminated soil, which is applied in the field of microbial remediation of heavy metal-contaminated soil. It can solve problems that have not been reported, achieve good remediation effect, reduce plant accumulation, and improve the existing form.

Active Publication Date: 2011-11-16
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there have been studies abroad on the adsorption of heavy metal cadmium in water by Rhodobacter sphaeroides, and some domestic scholars have used Rhodobacter sphaeroides to treat mine wastewater, and achieved good removal effects, but there has been no report on the restoration of heavy metal-contaminated soil by Rhodobacter spheroides. Compared with the traditional immobilization method, the microbial immobilization method has the characteristics of low cost, good effect and no secondary pollution

Method used

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  • Method for repairing microorganisms in heavy metal contaminated soil
  • Method for repairing microorganisms in heavy metal contaminated soil
  • Method for repairing microorganisms in heavy metal contaminated soil

Examples

Experimental program
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Effect test

Embodiment 1

[0043] A microbial remediation method for heavy metal-contaminated soil is provided in this embodiment, which specifically includes the following steps:

[0044] Step 1: Isolation of Rhodobacter sphaericus:

[0045] Rhodobacter sphaericus was isolated from oil flooding water by coating and line-drawing separation methods. The isolated bacteria were identified as Rhodobacter sphaericus by 16sRNA gene sequence determination data and cell morphology and physiological and biochemical experimental data.

[0046] Rhodobacter sphaeroides isolated from oil flooding water in northern China oilfields by coating and line-drawing separation methods are as follows:

[0047] (1) Add 100ml of sterilized liquid culture medium to the Erlenmeyer flask, then add 1ml of oilfield oil flooding water injection, and cultivate for 2 days at 25°C to obtain a medium with oilfield oil flooding water injection, repeat the above process repeatedly , A total of 3 bottles of medium supplemented with oilfiel...

Embodiment 2

[0060] This embodiment provides a microbial remediation method for heavy metal-contaminated soil, which specifically includes the following steps:

[0061] Step 1: Isolation of Rhodobacter sphaericus:

[0062] Rhodobacter sphaeroides were isolated from oil flooding water by coating and line-drawing separation methods. The isolated bacterium is identified as Rhodobacter sphaericus by 16sRNA gene sequence determination data and cell morphology and physiological and biochemical experimental data.

[0063] Bacteria isolated from oil flooding water in northern China oilfields by coating and line-drawing separation methods are as follows:

[0064] (1) Add 100ml of sterilized liquid culture medium to the Erlenmeyer flask, then add 2ml of oilfield oil flooding water injection, and cultivate at 35°C for 3 days to obtain a culture medium added with oil field oil flooding water injection, repeat the above process repeatedly , a total of 5 bottles of medium supplemented with oilfield oi...

Embodiment 3

[0076] This embodiment provides a microbial remediation method for heavy metal-contaminated soil, which specifically includes the following steps:

[0077] Step 1: Isolation of Rhodobacter sphaericus:

[0078] Rhodobacter sphaericus was isolated from oil flooding injection water by coating and line-drawing separation methods. The isolated bacteria were identified as Rhodobacter sphaericus by 16sRNA gene sequence determination data and cell morphology, physiological and biochemical experimental data.

[0079] Rhodobacter sphaeroides isolated from oil flooding water in northern China oilfields by coating and line-drawing separation methods are as follows:

[0080] (1) Add 100ml of sterilized liquid medium into the Erlenmeyer flask, then add 1.5ml of oilfield oil flooding water injection, and cultivate it for 2 days at 30°C to obtain a culture medium added with oil field oil flooding water injection, and repeat the above During the process, a total of 4 bottles of medium supplem...

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Abstract

The invention provides a method for repairing microorganisms in heavy metal contaminated soil, specifically comprising the following steps of: step 1, separating rhodobacter sphaeroides; step 2, culturing the rhodobacter sphaeroides; and step 3, using the rhodobacter sphaeroides to repair the heavy metal contaminated soil. By means of the method for repairing the microorganisms in the heavy metalcontaminated soil provided by the invention, the existence shapes of heavy metals in the soil can be improved effectively; and the plant accumulations of the heavy metals are greatly reduced. The method for repairing the microorganisms in the heavy metal contaminated soil disclosed by the invention belongs to in-situ repairing and has the advantages of simple operation, low cost and good repairing effect. Furthermore, the soil fertility is not damaged; and the secondary contamination of the environment does not exist.

Description

technical field [0001] The invention belongs to the field of soil microbial remediation, and in particular relates to a microbial remediation method for heavy metal polluted soil. Background technique [0002] Soil heavy metal pollution has the characteristics of poor mobility of pollutants in the soil, long residence time, and inability to be degraded by microorganisms, and can eventually affect human health through water, plants and other media. The "bone pain disease" and "Minamata disease" that occurred in history are typical cases of diseases caused by heavy metal pollution. Facing such a serious problem of heavy metal pollution in soil, it is urgent to develop a feasible treatment technology. [0003] In recent years, people have paid more and more attention to the problem of soil pollution. In order to reduce the pollution of heavy metals to soil, people have done a lot of research and developed various treatment technologies. One is activation, which mainly uses dif...

Claims

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Application Information

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IPC IPC(8): B09C1/10
Inventor 范文宏贾莹莹姜维吴矿
Owner BEIHANG UNIV
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