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Preparation of fluorescent silicon dioxide nanospheres

A silicon dioxide and nanosphere technology, applied in the field of nanomaterial technology and biological detection, can solve the problems of weakened fluorescence intensity, the fluorescence performance of fluorescent spheres is easily affected by the environment, and fluorescence self-quenching, etc. Uniform appearance and low price

Inactive Publication Date: 2011-11-09
张永昶
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AI Technical Summary

Problems solved by technology

The fluorescence properties of the fluorescent balls prepared by this method are easily affected by the environment, and the dyes and dye molecules are in contact with each other, and it is easy to produce fluorescence self-quenching due to too many molecules, which weakens the fluorescence intensity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] Weigh 4 mg of fluorescein isothiocyanate, place it in a single-necked flask, add 4 mL of absolute ethanol, and stir to dissolve the fluorescein isothiocyanate to form a uniform solution.

[0024] Add 60ul of aminopropyltrimethoxysilane to the solution, and stir the reaction at room temperature in the dark for 12h.

[0025] Take 1ml of the reaction solution, add it into a 100ml three-neck flask, add 20ml of absolute ethanol, and 2ml of ammonia water and stir evenly.

[0026] Add 1ml of ethyl orthosilicate and stir vigorously at room temperature for 5h.

[0027] The obtained reaction solution was centrifuged, the supernatant was poured off, the precipitate was washed with ethanol and then centrifuged, and this was repeated 4 times.

[0028] The precipitate obtained after washing was dried in a vacuum drying oven at a constant temperature of 60° C. for 3 hours to obtain the required silica fluorescent nanospheres.

[0029] The size of the silica fluorescent nanospheres o...

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Abstract

The invention provides fluorescent silicon dioxide nanospheres prepared at normal temperature and normal pressure by attempting to use a method of combining a dye with a silane coupling agent and then carrying out copolycondensation together with ethyl orthosilicate for the first time. According to the invention, the production method is simple, the cost is low and the reaction rate is high; and the fluorescent silicon dioxide nanospheres prepared by the invention have the advantages of uniform morphology and adjustable particle size and fluorescence intensity. The size of the obtained fluorescent silicon nanospheres is about 50-80 nm. The application of silicon dioxide nanomaterials in the fields of chemical synthesis, biological medicine, electronics, optics and other fields attracts wide attention. Because of non-toxicity, biocompatibility and easiness for introduction of fluorescent dyes, the silicon dioxide nanospheres become good biological fluorescent nanoparticles and can be widely used in biomedical imaging and immunoassays.

Description

[technical field] [0001] The invention relates to a nanometer material technology and the field of biological detection, in particular to a method for preparing silica nanospheres with fluorescence characteristics. [Background technique] [0002] The analysis and detection of trace active substances in the living body are of great significance for obtaining chemical and biological information in the life process, understanding the relationship between biomolecules and their structures and functions, explaining the mechanism of life activities and diagnosing diseases. Bioluminescent nanoparticles The successful development of bioluminescent nanoparticles provides conditions for the best single-molecule research in complex environments. The particle size of bioluminescent nanoparticles is 1-100nm, which has a huge specific surface area, size quantum effect and chemical reactivity, making it compatible with biological The body has a special interaction and has been widely used....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06
Inventor 张永昶
Owner 张永昶
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