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Liquid chromatography-tandom mass spectrometry detection method of thirty-one drugs in human blood

A liquid chromatography and tandem mass spectrometry technology, applied in the field of liquid chromatography-tandem mass spectrometry detection of 31 kinds of drugs in human blood, can solve the problem of high false positive rate, achieve good overall recovery rate, simplify the pretreatment process, and good linearity Effect

Inactive Publication Date: 2013-06-12
LIANYUN PORT IMMIGRATION INSPECTION & QUARANTINE BUREAU PEOPLES REPUBLIC OF CHINA
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  • Description
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Problems solved by technology

At present, the inspection and quarantine department mainly uses colloidal gold technology to quickly detect morphine and methamphetamine in the urine of entry-exit personnel. The false positive rate is extremely high, and only two kinds of drugs can be detected.

Method used

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  • Liquid chromatography-tandom mass spectrometry detection method of thirty-one drugs in human blood
  • Liquid chromatography-tandom mass spectrometry detection method of thirty-one drugs in human blood
  • Liquid chromatography-tandom mass spectrometry detection method of thirty-one drugs in human blood

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Embodiment 1

[0048] Example 1. The method for detecting 31 drugs in human blood by liquid chromatography-tandem mass spectrometry has the following steps:

[0049] (1) Sample treatment: Weigh 2g sample, accurate to 0.01g, and place it in a 50mL centrifuge tube with stopper. First add 1mL of 0.1mol / L NaOH solution, then add 10mL of ethyl acetate, and place it on a liquid mixer. Mix for 1 min, centrifuge at 4000r / min for 10 min, pipette the upper layer of ethyl acetate into a test tube; add 0.2mL 1.0mol / L NaH 2 PO 4 Then, after mixing thoroughly on a liquid mixer, add 10 mL of ethyl acetate to repeat the extraction once, and combine the two extracts; the extract is blown dry with a nitrogen dryer at 40°C; 1 mL of volume ratio 30:70 is added After vortexing methanol: water and passing through a 0.25μm filter membrane, the sample to be tested is obtained;

[0050] (2) Determination: The sample to be tested is determined by liquid chromatography-tandem quadrupole mass spectrometer; in the liquid ch...

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Abstract

The invention relates to a liquid chromatography-tandom mass spectrometry detection method of common drugs in human blood. According to the method of the invention, an extraction method, a purification method, chromatographic conditions and mass spectral conditions of the thirty-one common drugs in the human blood are established. The method has a minimum detection limit of 1 to 2 ng / mL. When the concentration is from 2 to 100 ng / ml, the linearity is good with a related coefficient of 0.9771 to 0.9995 and an RSD of less than 13.2%. The method of the present invention has the advantages of strong pertinence, simple operation, fast detection speed, wide detection area, and easy popularization and application, can be used for fast detection of entry-exit inspection and quarantine departments, disease control centers and police departments, and positive result confirmation. The drugs can be detected through blood sampling, so a detection escape phenomenon which may appear when entry-exit inspection and quarantine systems guard passes at ports is prevented.

Description

Technical field [0001] The invention relates to a method for detecting drugs, in particular to a method for detecting 31 drugs in human blood by liquid chromatography-tandem mass spectrometry. Background technique [0002] Drug abuse and drug trafficking have become a global social hazard. Once people are contaminated with drugs, they will often indulge in the abnormal "pleasure" imagined by drugs, and have no intention of working and studying. Drug abuse can also cause a series of family and social problems. In view of the serious harm to the family, especially to the society, from taking drugs, some foreign countries, in addition to adopting corresponding management measures against drug users in their own countries, also require the applicants to provide proof that the applicant does not take drugs, such as Russia. As the process of global economic integration accelerates, other countries will inevitably introduce similar regulations for immigrants. In order to actively res...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 张睿王海涛吴斌赵建陶宏锦丁永健姚燕林王学虎赵武生王绪发王维志段宏安刘利刘建徐聪林徐文科邵景东
Owner LIANYUN PORT IMMIGRATION INSPECTION & QUARANTINE BUREAU PEOPLES REPUBLIC OF CHINA
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