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Liquid chromatography-tandom mass spectrometry detection method of thirty-one drugs in human blood

A liquid chromatography and tandem mass spectrometry technology, which is applied in the field of liquid chromatography-tandem mass spectrometry detection of 31 kinds of drugs in human blood, can solve the problem of high false positive rate, achieve good overall recovery rate, good purification effect, and simplify pretreatment The effect of the process

Inactive Publication Date: 2011-10-26
LIANYUN PORT IMMIGRATION INSPECTION & QUARANTINE BUREAU PEOPLES REPUBLIC OF CHINA
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Problems solved by technology

At present, the inspection and quarantine department mainly uses colloidal gold technology to quickly detect morphine and methamphetamine in the urine of entry-exit personnel. The false positive rate is extremely high, and only two kinds of drugs can be detected.

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  • Liquid chromatography-tandom mass spectrometry detection method of thirty-one drugs in human blood
  • Liquid chromatography-tandom mass spectrometry detection method of thirty-one drugs in human blood
  • Liquid chromatography-tandom mass spectrometry detection method of thirty-one drugs in human blood

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Embodiment 1

[0048] Example 1. The liquid chromatography-tandem mass spectrometry detection method of 31 kinds of drugs in human blood, the steps are as follows:

[0049] (1) Sample treatment: Weigh 2g sample, accurate to 0.01g, put it into a 50mL stoppered centrifuge tube, first add 1mL 0.1mol / L NaOH solution, then add 10mL ethyl acetate, and fully Mix well for 1min, centrifuge at 4000r / min for 10min, absorb the upper layer of ethyl acetate into the test tube; add 0.2mL 1.0mol / LNaH 2 PO 4 Finally, after fully mixing on a liquid mixer, add 10mL of ethyl acetate to repeat the extraction once, and combine the two extracts; the extract is dried at 40°C with a nitrogen blower; add 1mL of After methanol: water vortex, after passing through a 0.25 μm filter membrane, the sample to be tested was obtained;

[0050] (2) Determination: The sample to be tested is measured with a liquid chromatography-tandem quadrupole mass spectrometer; in the liquid chromatography-tandem mass spectrometry analysi...

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Abstract

The invention relates to a liquid chromatography-tandom mass spectrometry detection method of common drugs in human blood. According to the method of the invention, an extraction method, a purification method, chromatographic conditions and mass spectral conditions of the thirty-one common drugs in the human blood are established. The method has a minimum detection limit of 1 to 2 ng / mL. When the concentration is from 2 to 100 ng / ml, the linearity is good with a related coefficient of 0.9771 to 0.9995 and an RSD of less than 13.2%. The method of the present invention has the advantages of strong pertinence, simple operation, fast detection speed, wide detection area, and easy popularization and application, can be used for fast detection of entry-exit inspection and quarantine departments, disease control centers and police departments, and positive result confirmation. The drugs can be detected through blood sampling, so a detection escape phenomenon which may appear when entry-exit inspection and quarantine systems guard passes at ports is prevented.

Description

technical field [0001] The invention relates to a drug detection method, in particular to a liquid chromatography-tandem mass spectrometry detection method for 31 kinds of drugs in human blood. Background technique [0002] Drug abuse and drug trafficking have become a global social hazard. Once a person is infected with drugs, he will often indulge in the deformed "pleasure" imagined by drugs, and have no intention of working and studying. Drug use can also cause a series of family and social problems. In view of the serious harm of drug use to the family, especially to the society, some foreign countries, in addition to taking corresponding management measures for their own drug users, also require applicants to provide proof of non-drug use, such as Russia. As the process of global economic integration accelerates, it is inevitable that other countries will introduce similar regulations for immigrants one after another. In order to actively respond to the requirements ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 张睿王海涛吴斌赵建陶宏锦丁永健姚燕林王学虎赵武生王绪发王维志段宏安刘利刘建徐聪林徐文科邵景东
Owner LIANYUN PORT IMMIGRATION INSPECTION & QUARANTINE BUREAU PEOPLES REPUBLIC OF CHINA
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