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Culturing method of cordyceps militaris rich in effective component (schisandra chinensis)

A technology of active ingredients and cultivation methods, which is applied in the field of cultivation of Cordyceps militaris, can solve the problems of large doses and inconvenient medicines, and achieve the effect of simplifying methods, easy implementation conditions, and increasing the content of cordycepin and adenosine

Inactive Publication Date: 2011-10-26
王淑华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In Chinese medicine, Cordyceps militaris is often used in combination with various tonic Chinese medicines to achieve synergistic effects. However, when patients use traditional Chinese medicines in combination, they often stop using the medicines due to the large dosage and the inconvenience of taking them.

Method used

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  • Culturing method of cordyceps militaris rich in effective component (schisandra chinensis)
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  • Culturing method of cordyceps militaris rich in effective component (schisandra chinensis)

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specific Embodiment approach 1

[0011] Specific Embodiment 1: In this embodiment, the culture method of Cordyceps militaris rich in active ingredients of Schisandra chinensis is carried out according to the following steps: 1. Inoculate the Cordyceps militaris strain Yufeng No. 1 on the PDA solid medium, and culture in dark at 23°C for 7 days , to obtain the activated bacterial classification; 2, cut the activated bacterial classification into 1cm × 1cm bacterium pieces and inoculate them in the liquid seed medium of 300ml, and cultivate them darkly for 7d under the condition of 23°C and 120r / min at a temperature to obtain Liquid strains; 3. Break the liquid strains with a magnetic stirrer and dilute them 250 times, then take 15ml and inoculate them into the solid culture medium of Cordyceps militaris rich in Schisandra chinensis. After the film is sealed, the temperature is 22°C and the humidity is 55%. Under the condition of ~60% dark culture for 6 ~ 9 days, mycelium was obtained, and then cultured under th...

specific Embodiment approach 2

[0019] Specific embodiment two: what this embodiment is different from specific embodiment one is that the PDA solid culture medium is made up of the potato liquid of 200g, the glucose of 20g, the agar of 18g and the water of 1000ml in the step one, and pH is natural; Preparation: 200g of peeled potatoes, cut into pieces and boiled for 30 minutes, filtered through gauze to make up to 1000ml, with natural pH, autoclaved at 121°C for 30 minutes. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0020] Specific embodiment three: what this embodiment is different from specific embodiment one is that in step 2, every L of liquid seed culture medium is made up of the water of the potato liquid of 200g, the glucose of 20g, the magnesium sulfate of 1g, 1g potassium dihydrogen phosphate and surplus Composition, natural pH; preparation of the potato liquid: 200g peeled potatoes, cut into pieces and boiled for 30 minutes, filtered through gauze to make up to 1000ml, natural pH, autoclaved at 121°C for 30 minutes. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention discloses a culturing method of cordyceps militaris rich in effective component (schisandra chinensis) and relates a culturing method of cordyceps militaris, and the method can be used for simplifying traditional Chinese medicine for compatibility, and improving the utilization rate of the cordyceps militaris. The method comprises the following steps: 1. activating a strain; 2. preparing a liquid strain; 3. smashing and diluting the liquid strain, and inoculating the smashed and diluted strain to a solid culturing medium of the cordyceps militaris rich in schisandra chinensis, and carrying out dark culture to obtain a mycelium; culturing the mycelium under illumination to obtain a mature sporocarp; continuously culturing until a stroma grows to 4-8cm and an orange power substance appears on the surface of the stroma; and harvesting. The method provided by the invention has the following advantages: based on the mutual effect of the components in the schisandra chinensis and cordyceps sinensis growth metabolism, the contents of adenosine and cordycepin are improved, and simultaneously a cordyceps sinensis sporocarp with an effective component (schisandra chinensis) is generated, thereby playing roles in utilizing the cordyceps militaris and carrying out compatibility on the cordyceps militaris, simplifying the compatible method of the schisandra chinensis and the cordyceps militaris, and improving the nutrition and medical value of the schisandra chinensis.

Description

technical field [0001] The invention relates to a method for cultivating Cordyceps militaris. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris [C.militarise (L.) Link], Cordyceps militaris, belongs to Ascomycotina (Asco mycotina) Sclerotinia (pyrenomycetes) Sphaerinles (Sphaerinles) Ergotaceae (claviticpitaceae) Cordyceps genus (Codyceps), is one of the precious Chinese herbal medicines in my country. It has various functions such as hypnosis and sedation, nourishing the liver and kidney, tonifying deficiency, preventing cancer, anti-cancer, hemostasis, reducing phlegm, and relieving asthma. In "Materia Medica Congxin", it is recorded that "Ganping protects the lungs, nourishes the kidneys, nourishes the essence, stops bleeding and resolves phlegm" and other curative effects. Because Cordyceps enters the two meridians of the lung and kidney, it can not only nourish the lung yin, but also nourish the kidney yang. It is the only traditional...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
Inventor 王淑华杨鹏王双平张文艳袁斯文
Owner 王淑华
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