Corn breeding method

Abstract

The invention provides a corn breeding method, comprising the following steps: radiating seeds of a second cycle corn or a population mixture, then planting, selecting target character variation strains for inbreeding, carrying out single ear threshing, planting again and inducing with an inducer, and selecting haploid grains with a purple color label on top of an aleurone layer, and without a purple label on embryonic tips; doubling the haploid grains, carrying out inbreeding on the successfully doubled plants, and collecting inbred maturing grains; planting the selfing maturing grains, selecting ears satisfying breeding object requirements for inbreeding, obtaining a new corn inbred line, and testing the new corn inbred line pollen with testing seeds; planting the testing combination, carrying out field identification and finishing breeding a new corn species. The method combines the haploid breeding and the mutation breeding to rapidly obtain a homozygous inbred line for assembling a hybrid combination, so as to reduce breeding time and raise correctness and efficiency of corn variety breeding.

Description

technical field [0001] The invention relates to a method for cultivating plant varieties, in particular to a method for breeding maize by combining ray radiation mutagenesis and haploid induction. Background technique [0002] Radiation mutation breeding refers to the use of various rays, such as X-rays, γ-rays, neutrons, etc. to irradiate the seeds, plants or certain organs and tissues of crops to promote them to produce various variations, and then select the required heritable good variations , a breeding method for cultivating new elite varieties. At present, radiation mutation breeding has been widely used in self-breeding crops such as a variety of vegetables and wheat; the advantage of this breeding method is that it can increase the frequency of variation and enrich the species quality resources; the main problem of mutation breeding is that the frequency of beneficial mutations is still low, and the direction and nature of variation are still difficult to control. T...

AI Technical Summary

Problems solved by technology

[0002] Radiation mutation breeding refers to the use of various rays, such as X-rays, γ-rays, neutrons, etc. to irradiate the seeds, plants or certain organs and tissues of crops to promote them to produce various variations, and then select the required heritable good variations , a breeding method for cultivating new elite varieties. At present, radiation mutation breeding has been widely used in self-breeding crops such as a variety of vegetables and wheat; the advantage of this breeding method is that it can increase the frequency of variation and enrich the species quality resources; the main problem of mutation breeding is that the frequency of beneficial mutations is still low, and the direction and nature of variation are still difficult to control. Therefore, if direct radiation is used to mutate maize inbred lines, there are often not many beneficial individuals after mutation, and it is difficult to To obtain more abundant beneficial variation, if radiation mutagenesis segregates materials such as maize dicyclic lines or mixed populations with richer types, multiple generations of selfing selection are required to obtain homozygous inbred lines, and then hybrids can be assembled. The breeding years are long, and the advantages of radiation mutation breeding have not been well utilized, which is the main reason why radiation mutation breeding is less used in maize breeding