DHA (docosahexaenoic acid)-containing grain blend oil and preparation method thereof

A technology for blending oils and grains, applied in food science, edible oil/fat, applications, etc., can solve problems such as acquisition, and achieve the effect of avoiding pollution and reducing fishy smell

Active Publication Date: 2011-10-05
SHANDONG SANXING CORN IND SCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above reasons make it impossible for most people to obtain enough DHA through daily diet alone

Method used

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  • DHA (docosahexaenoic acid)-containing grain blend oil and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Schizochytrium fermentation

[0034] a) Strain activation → primary fermentation → secondary fermentation → tertiary fermentation: use liquid culture medium, the main ingredients are 2% corn starch plus 0.2% yeast powder, and also contain sodium glutamate 2g / L, vitamin B 1 10ug / L, Vitamin B 12 1ug / L, adjust the pH to 6±0.2 with artificial seawater, and then sterilize by high pressure steam at 121°C for 15min. Schizochytrium was used as the strain, inoculated with 5% inoculum in the medium configured above, and cultured for 5 days at 28°C and 170r / min shaker light culture conditions.

[0035] (2) Purification of DHA

[0036] a) Centrifuge the Schizochytrium fermented liquid with a disc centrifuge to separate the cells at a speed of 2500r / min for 25min, and vacuum dry and dehydrate at 105°C for 5 hours under an absolute pressure of 0.1MP;

[0037] b) Add 1.5ml of distilled water and 2.0ml of concentrated hydrochloric acid to the dried bacteria at a rate of 0.5 g...

Embodiment 2

[0045] (1) Schizochytrium fermentation

[0046] a) Strain activation → primary fermentation → secondary fermentation → tertiary fermentation; liquid medium is used, the main component is 2% corn starch plus 0.2% yeast powder, and it also contains sodium glutamate 1.5g / L, Vitamin B 1 10ug / L, Vitamin B 12 1 ug / L, adjust the pH to 6±0.2 with artificial seawater, and then sterilize by high pressure steam at 121°C for 15 minutes. Schizochytrium was used as the strain, inoculated with 5% inoculum in the medium configured above, and cultured for 5 days at 26°C and 170r / min shaker light culture conditions.

[0047] (2) Purification of DHA

[0048] a) Centrifuge the Schizochytrium fermented liquid with a disc centrifuge to separate the cells at a speed of 2500r / min for 25min, vacuum-dry and dehydrate at 105°C for 5 hours at an absolute pressure of 0.1MP;

[0049] b) Add 1.5ml of distilled water and 2.0ml of concentrated hydrochloric acid to the dried bacteria at a rate of 0.5 gram...

Embodiment 3

[0054] (1) Schizochytrium fermentation

[0055] a) Strain activation → primary fermentation → secondary fermentation → tertiary fermentation; liquid medium is used, the main ingredients are 2% corn starch plus 0.2% yeast powder, and it also contains sodium glutamate 2g / L, vitamin B 1 10ug / L, Vitamin B 12 1ug / L, adjust the pH to 6±0.2 with artificial seawater, and then sterilize by high pressure steam at 121°C for 15min. Schizochytrium was used as the strain, inoculated with the inoculum amount of 5% in the medium configured above, and cultured for 5 days at 27°C and 170r / min shaker light culture conditions.

[0056] (2) Purification of DHA

[0057] a) Centrifuge the Schizochytrium fermented liquid with a disc centrifuge to separate the cells at a speed of 2500r / min for 25min, vacuum-dry and dehydrate at 105°C for 5 hours at an absolute pressure of 0.09MP;

[0058] b) Add 1.5ml of distilled water and 2.0ml of concentrated hydrochloric acid to the dried bacteria for every 0...

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Abstract

The invention belongs to the field of edible oil products and preparation method of edible oil and particularly relates to DHA (docosahexaenoic acid)-containing grain blend oil and a preparation method thereof. The DHA-containing grain blend oil is characterized by comprising the following components: DHA, soybean oil and corn oil. According to the invention, since the grain blend oil contains DHA, people can acquire enough DHA from daily diet, rather than acquiring DHA from marine products or aquatic products; in addition, as used DHA is from schizochytrium aggregatum which does not contain EPA (eiscosapentaenoic acid), thus avoiding marine pollution and having small fishy smell. The corn oil contains more than 65 percent of linoleic acid, 80-120mg / 100g VE and more than 500mg / 100g phytosterol, and the soyabean oil also is rich in vitamins. The DHA-containing grain blend oil can provide enough nutrition and DHA for all people.

Description

technical field [0001] The invention belongs to the field of edible oil products and a preparation method thereof, and in particular relates to DHA-containing grain blended oil and a preparation method thereof. Background technique [0002] DHA (docosahexaenoic acid) is a long-chain omega-3 fatty acid distributed throughout the body. More specifically, it is the main building fat of the brain and eyes and an important component of the heart. Accumulating research data show that DHA plays a very important role in maintaining good health in all stages of life. [0003] To maintain the normal functions of various tissues, the human body must ensure that there are sufficient fatty acids. If they are deficient, they can cause a series of symptoms, including growth retardation, abnormal skin scales, mental retardation, etc. As an essential fatty acid, DHA has more significant effects on enhancing memory and thinking ability and improving intelligence. Population epidemiological...

Claims

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Application Information

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IPC IPC(8): A23D9/007A23D9/04
Inventor 王明星王月华
Owner SHANDONG SANXING CORN IND SCI CO LTD
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