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Method for rapidly and effectively acquiring umbilical cord mesenchymal stem cell (MSC)

A fast and umbilical cord technology, applied in the direction of animal cells, vertebrate cells, bone/connective tissue cells, etc., can solve the problems of slow speed and low acquisition efficiency

Inactive Publication Date: 2011-09-21
侯亚义
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  • Application Information

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Problems solved by technology

Relevant literature reports the method of obtaining umbilical cord MSC cells, but the obtaining efficiency is relatively low and the speed is slow

Method used

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  • Method for rapidly and effectively acquiring umbilical cord mesenchymal stem cell (MSC)
  • Method for rapidly and effectively acquiring umbilical cord mesenchymal stem cell (MSC)
  • Method for rapidly and effectively acquiring umbilical cord mesenchymal stem cell (MSC)

Examples

Experimental program
Comparison scheme
Effect test

example 3

[0034] Example 3 Determination of umbilical cord MSC cell yield and cell growth curve.

[0035] After the obtained MSC cells were digested with cell digestion solution (0.25% trypsin+0.02% EDTA) on the eighth day of the first generation, viable cells were counted with 0.4% placepan blue, and the yield of the first generation cells was counted. After digesting the third-generation cells with cell digestion solution (0.25% trypsin + 0.02% EDTA), they were seeded in a 24-well plate at 1×104 cells / well, digested 3 wells every 24 hours, collected the cells, and used 0.4 % placepan blue count live cells, draw growth curve. The first three generations of cells grow slowly, so they need to be inoculated and passaged at a ratio of 1:1. From the fourth generation, the cells proliferate faster. In the logarithmic growth phase, the cell doubling time is about 36 hours. Each generation of cells, the number of cells increased by about 2 times. It is basically consistent with the reports i...

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Abstract

The invention belongs to the field of biological medicines and discloses a method for rapidly and effectively acquiring an umbilical cord mesenchymal stem cell (MSC). The method is characterized in that the umbilical cord MSC is rapidly and effectively acquired by taking the umbilical cord of a newly born fetus as a resource and is rapidly identified by using an optimized umbilical cord tissue digestive enzyme formula and a digestive cell adherence mode. The method is developed by improvement based on the traditional MSC, the time for acquiring the MSC is shortened to the half of the traditional method, the yield is improved by one time, and various surface characteristics and functions of the MSC are not influenced. The invention provides the method for rapidly and effectively acquiring the MSC for the scientific researches and clinical application of the umbilical cord MSC.

Description

Technical field: [0001] The present invention is a method for quickly and effectively obtaining umbilical cord MSCs, specifically a method for improving the yield and speed of obtaining umbilical cord MSCs by using cell culture technology and reasonable tissue processing technology, and quickly identifying them. Background technique [0002] Mesenchymal stem cells are a type of pluripotent stem cells belonging to the mesoderm, which mainly exist in connective tissue and organ mesenchyme. MSCs have strong proliferative ability and multi-directional differentiation potential, and can differentiate into hematopoietic cells, muscle cells, liver cells, osteoblasts, chondrocytes, stromal cells and other cells under suitable in vivo or in vitro environments. At the same time, in addition to the general biological characteristics of stem cells, MSCs also have immune regulation functions. In addition, the cells have a relatively convenient source, are easy to separate, cultivate, ex...

Claims

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Application Information

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IPC IPC(8): C12N9/00C12N9/48C12N9/50C12N9/26C12N5/0775
Inventor 侯亚义刘柳李鹏飞赵晓寅赵叶琳王亚平
Owner 侯亚义
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