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Bone peptide composition, preparation thereof, preparation method thereof and application

A technology of composition and osteopeptide, which is applied in the field of preparation, osteopeptide composition and preparation thereof, can solve the problems of long extraction process time, influence of extract biological activity, etc.

Active Publication Date: 2011-09-21
黑龙江三元阳普医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patented extraction method uses high-pressure extraction, and the extraction process takes a long time, which will affect the biological activity of the extract

Method used

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  • Bone peptide composition, preparation thereof, preparation method thereof and application
  • Bone peptide composition, preparation thereof, preparation method thereof and application
  • Bone peptide composition, preparation thereof, preparation method thereof and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] A method for preparing an osteopeptide composition includes the following steps:

[0078] 1. Take healthy fresh frozen pork limb bones, wash and crush;

[0079] 2. Grind the crushed limb bones into bone mud with a bone mud machine, put it in an extraction tank, add 3 times the amount of water, heat and stir at 80°C for 2 hours, take the supernatant and store; add 2 times the amount of the obtained bone residue Heat and stir water at 80°C for 1 hour, take the supernatant; combine the supernatants obtained twice and concentrate under reduced pressure at 60°C to the weight of the raw material;

[0080] 3. Place the solution obtained in step 2 at 0°C for 12 hours to remove the upper layer of fat;

[0081] 4. The solution is adjusted to pH 4.5 with hydrochloric acid, heated and boiled for 15 minutes, filtered, and cooled, placed at 0°C for 12 hours, and centrifuged after removing the upper layer of fat; the centrifugal condition is 2000 rpm and the time is 30 minutes;

[0082] 5. The...

Embodiment 2

[0090] A method for preparing an osteopeptide composition includes the following steps:

[0091] 1. Take the limb bones of healthy and freshly frozen pigs, wash and crush;

[0092] 2. The crushed limb bones are ground into bone mud with a bone mud machine. The bone mud is microwaved. The microwave power is 450W and the action time is 5 minutes. Then put it into the extraction tank, add 6 times the amount of water, and keep it warm at 100°C. Stir for 1 hour, take the supernatant and store; add 4 times the amount of water to the obtained bone residue, keep stirring at 100°C for 1 hour, and take the supernatant; combine the supernatant obtained twice and concentrate under reduced pressure at 60°C to the weight of the raw material;

[0093] 3. Place the solution obtained in step 2 at 0°C for 12 hours to remove the upper layer of fat;

[0094] 4. The solution is adjusted to pH 4.5 with hydrochloric acid, heated and boiled for 20 minutes, filtered and cooled, and placed at 0°C for 12-24 hou...

Embodiment 3

[0103] A method for preparing an osteopeptide composition includes the following steps:

[0104] 1. Take the limb bones of healthy freshly frozen fetal cattle, wash and crush;

[0105] 2. The crushed limb bones are ground into bone mud with a bone mud machine. The bone mud is microwaved. The microwave power is 450W and the action time is 15 minutes. Then put it into the extraction tank, add 4 times the amount of water, and keep it warm at 90°C. Stir for 1.5 hours, take the supernatant and store; add 3 times the amount of water to the obtained bone residue, keep at 90°C and stir for 1.5 hours, and take the supernatant; combine the supernatant obtained twice and concentrate under reduced pressure at 60°C to the weight of the raw material;

[0106] 3. Place the solution obtained in step 2 at 0°C for 18 hours to remove the upper layer of fat;

[0107] 4. The solution is adjusted to pH 5.0 with hydrochloric acid, heated and boiled for 20 minutes, filtered, cooled, and placed at -5°C for 18...

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Abstract

The invention relates to a bone peptide composition, which comprises the following components in part by weight: 8 to 10 parts of bond polypeptide compound A, 5 to 8 parts of bone polypeptide compound B, 5 to 8 parts of bone polypeptide compound C, 4.5 to 7.5 parts of bone polypeptide compound D, 3 to 6 parts of bone polypeptide compound E, 16 to 22 parts of free amino acid, 0.3 to 5.4 parts of ribose, 0.04 to 0.12 parts of trace element and 0 to 0.01 parts of free fatty acid, wherein amino acid sequences of the bone polypeptide compounds A, B, C, D and E are measured. The invention also relates to a preparation method and application of the bone peptide composition. The preparation method is simple, convenient, easy to operate and high in yield, and the prepared bone peptide composition is high in bioactivity.

Description

Technical field [0001] The invention relates to a pharmaceutical composition, in particular to an osteopeptide composition and its preparation, preparation method and application. Background technique [0002] The extract extracted from animal bones, whose main components are small molecule peptides, is a mixture of small molecule peptides derived from the breakdown of collagen, which can regulate bone metabolism, stimulate osteoblast proliferation, promote new bone formation, and Regulate calcium and phosphorus metabolism, increase bone calcium deposition, and prevent osteoporosis. Clinically, it is mainly used to promote fracture healing. Among them, polypeptide extracts can directly act on osteogenic cartilage and osteoclasts by supplementing and increasing various osteogenic factors, promoting the synthesis of bone and cartilage matrix, and accelerating damage healing and repair; at the same time, polypeptide extracts in animal bones It can also promote the secretion of grow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/10A61K38/08C07K1/14C07K1/34A61P19/08A61P19/02A61P19/04A61P19/10A61P29/00A61K33/42A61K33/26A61K33/32A61K33/00A61K33/06A61K33/04
Inventor 俞嘉林丛艳权晓丹吴晶晶
Owner 黑龙江三元阳普医药有限公司
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