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Blueberry tissue culture method and special culture medium thereof

A tissue culture and culture medium technology, applied in the field of plant tissue culture, can solve the problems of large-scale industrial production and achieve the effects of shortening the culture cycle, consistent genetic traits, and low cost

Inactive Publication Date: 2011-09-21
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, domestic blueberry seedling breeding is still in its infancy, and there are many problems, and there are no large-scale factory production reports

Method used

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  • Blueberry tissue culture method and special culture medium thereof
  • Blueberry tissue culture method and special culture medium thereof
  • Blueberry tissue culture method and special culture medium thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1, preparation and statistical observation of "Heyun No. 1" regenerated plants

[0050] 1. Preparation and statistical observation of the first-generation tissue culture seedlings of "Heyun No. 1"

[0051] 1. Obtaining and pretreatment of explants

[0052] 1) Selection of explants

[0053] In this example, blueberry tissue culture adopts the mode of axillary bud proliferation. From June to September every year, in the "Heyun No. 1" blueberry seed resource nursery (purchased from Jilin Jikang Company), select blueberry seedlings with no pests and diseases, good tree shape, good fruit setting characteristics, and high yield to take explants. The explants are selected new shoots of that year, and there are 6-10 buds on one branch.

[0054] 2) Pretreatment of explants

[0055] Put the explants cut in the above step 1 into a beaker filled with clean water, drop 4-6 drops of detergent, gently scrub the axillary buds and stems of the branches with a soft brush, ...

Embodiment 2

[0097] Example 2, preparation and statistical observation of the first generation of tissue culture seedlings of "Heyun No. 1"

[0098] The difference between this example and Example 1 is that the final concentration of zeatin in the primary culture medium was adjusted to 4 mg / L, and the rest remained unchanged.

[0099] The statistical observations are as follows:

[0100] 1) Primary culture

[0101] When the stem section of blueberry with axillary buds in the primary culture medium of the above-mentioned embodiment 2 was cultured for 55-65 days, the blueberry tender shoots induced and differentiated could be observed. The experiment was repeated 3 times, and the induction situation of axillary buds was statistically observed when culturing for 60 days. The results are shown in Table 4 below, and the induction rate of buds can reach more than 93%.

[0102] Table 4. Induction rate of axillary buds

[0103] repeat

[0104] 2) Subculture

[0105] The first-genera...

Embodiment 3

[0109] Example 3, preparation and statistical observation of the first generation of tissue culture seedlings of "Heyun No. 2"

[0110] The difference between this example and Example 1 is that the stem segment with axillary buds of "Heyun No. 2" blueberry is used as explants for culture, and the NAA of the primary medium used is different from the ZT, NAA, and IBA of the secondary medium. , and the rest of the steps are consistent with those of Example 1.

[0111] In this example:

[0112] (1) "Heyun No. 2" blueberries were purchased from Jilin Jikang Company.

[0113] (2) The final concentration of NAA in the primary culture medium was 0.1mg / L.

[0114] (3) The final concentration of zeatin (ZT) in the subculture medium was 3 mg / L, the final concentration of NAA was 0.06 mg / L, and the final concentration of IBA was 0.3 mg / L.

[0115] The statistical observations are as follows:

[0116] 1) Primary culture

[0117] In the first-generation culture medium of the above-ment...

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Abstract

The invention discloses a blueberry tissue culture method and a special culture medium thereof. The special culture medium is a primary culture medium for blueberry tissue culture and is a solid culture medium which is obtained by adding zeatin, naphthyl acetic acid (NAA), a carbon source and gels into basic culture solution; and in the primary culture medium, the zeatin has the final concentration of 2-5mg / L, the NAA has the final concentration of 0.01-0.12mg / L, and the solvent of the basic culture solution is water and solute of the basic culture solution is shown as Table 1. Experiments prove that: the primary culture medium can greatly shorten the culture period of the tissue culture from three months to two months, the inductivity of sprouts is over 90 percent, and the production capacity of tissue culture each year is over 3 million seedlings. The method has the characteristics of high efficiency and time conservation, the produced tissue culture seedlings are low in cost, the average cost of each seedling is 1.5 yuan, the obtained blueberry tissue culture seedlings have consistent inherited characteristics, and the defect that the conventional reproduction coefficient is low is overcome.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for blueberry tissue culture and a special primary culture medium thereof. Background technique [0002] Blueberry (blueberry) scientific name lingonberry belongs to Ericaceae (Ericaceae) Vaccinium SPP. perennial shrubs. The fruit is a small blue-purple berry covered with a layer of white fruit powder. It is sweet and sour, with a unique flavor. It can be eaten fresh or deep-processed. It is rich in A, Vpp, Vp, and VB 1 , VB 2 , VB 5 , D, E, K and other vitamins, organic germanium, organic selenium and more than a dozen trace elements needed by the human body, as well as antioxidant components such as arbutin, anthocyanin, SOD, ellagic acid, and flavonoids, high Fiber, low in calories, and its nutritional value is much higher than that of ordinary fruits, especially the anthocyanin content of blue berries ranks first among all fruits and vegetables. Medical resear...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 孟凡乔吴文良李磊刘凝殷秀岩郭岩彬杨景娜
Owner CHINA AGRI UNIV
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