Method for preparing fermented high-lysine high-protein feed
A high-protein feed and high-lysine technology, which is applied in the field of preparation of high-lysine and high-protein feed, can solve the problem that the amino acid and protein content of the feed cannot meet the needs of animals, the investment in fixed assets is huge, and the post-processing process is troublesome, etc. problem, to achieve the effect of good animal absorption, good dispersibility, and improved physical and chemical properties
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[0019] The present invention relates to a method for preparing a fermented high-lysine high-protein feed, the preparation steps of which are as follows:
[0020] Step 1: After expanding the lysine-producing bacteria, when the number of viable bacteria is ≥ 5 billion / g, add bran of the same weight as the bacterial liquid, and ferment in the bran carrier. The fermentation time is 72-80 hours at 80°C Dried, crushed to a particle size of <2.5mm to obtain a lysine fermented product, and set aside;
[0021] Step 2: Cultivate Candida utilis for 36-48 hours using malt juice agar medium at a temperature of 30-35°C, a relative humidity of 40-50%, and a pH value of the medium of 5.0-6.0, and take it out Final drying, the drying temperature is 40-60°C, and final crushing until the particle size is less than 2.5mm to obtain the yeast fermentation product, which is used for later use;
[0022] Step 3: crush the soybean meal to a particle size of ≤1 mm, and set aside;
[0023] Step 4: Grin...
Embodiment 1
[0029] Step 1: After screening the lysine-producing corynebacterium, the screening includes activation and rejuvenation, and the dominant strains are selected for use. After that, the strains are expanded and cultivated through primary and secondary strains. When the number of viable bacteria reaches 5 billion / g, Add bran with the same weight as the bacterial liquid, ferment in the bran carrier, and stop the fermentation after 72 hours of fermentation. At this time, the acid production reaches 10%, and the number of viable bacteria reaches ≥10 billion / g. After the fermentation is completed, the lysine The content is 5.5%, dried at 80°C, and crushed to a particle size of <2.5mm to obtain a lysine fermented product, which is set aside;
[0030] Step 2: Cultivate Candida utilis, stop the cultivation after 36 hours, the number of viable yeast cells reaches 200 million / g, and the drying temperature is 40°C; crush the particle size to <2.5mm, and obtain a yeast fermented product for ...
Embodiment 2
[0039] Step 1: After screening the lysine-producing corynebacterium, the screening includes activation and rejuvenation, and the dominant strains are selected for use. After that, the strains are expanded and cultivated through primary and secondary strains. When the number of viable bacteria reaches 5.5 billion / g, Add bran with the same weight as the bacterial liquid, ferment in the bran carrier, and stop the fermentation after 75 hours of fermentation. At this time, the acid production reaches 12%, and the number of viable bacteria reaches ≥10 billion / g. After the fermentation is completed, the lysine The content is 6%, dried at 80°C, crushed to a particle size of <2.3mm, and the lysine fermented product is obtained, which is set aside;
[0040] Step 2: Cultivate Candida utilis, stop the cultivation after 42 hours, the number of viable yeast cells reaches 200 million / g, and the drying temperature is 50°C; the crushed particle size is less than 2.5mm, and the yeast fermented p...
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