Device and method for measuring particulate motion of turbid media by using dynamic speckle method

Abstract

The invention relates to a device and a method for measuring particulate motion of turbid media by using a dynamic speckle method. The device comprises a laser beam shaping contracting part, a forward scattering speckle field measuring part, a backward scattering speckle field measuring part, and a computer control and processing system. The method comprises the following steps: utilizing the device to record a forward scattering speckle field and a backward scattering speckle field generated by the particulate motion of turbid media and store the fields into the computer; according to the particle light scattering theory, choosing the forward scattering speckle field or backward scattering speckle field data; and analyzing the motion characteristic of the particulate, by utilizing a speckle chart characteristic value. The method is characterized by real time, dynamic and accuracy and can be widely applied to the fields such as medicaments, biology, life science, and the like.

Description

technical field [0001] The invention relates to a device and a method for measuring the movement of particles in a turbid medium, in particular to a device and a method for measuring the movement of particles in a turbid medium using a dynamic speckle method. Background technique [0002] Turbid medium refers to a medium with non-uniform refractive index that can produce both scattering and absorption and is mainly caused by scattering; most biological tissues (juice) contain a large number of scattering particles, which are typical turbid media. Therefore, the study of the motion characteristics of particles in turbid media has important academic value and scientific significance to the fields of medicine, biology, and life sciences, and is a frontier hotspot in modern scientific research. [0003] Speckle refers to the random intensity distribution of light and dark that is formed after coherent light is scattered by a rough surface or an inhomogeneous medium. When the irr...

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Problems solved by technology

[0004] After literature search, the U.S. patent "Two-dimensional imaging of translucent objects in turbid media" (authorization number US5644429A, authorization date 1997.07.01) provides a 4F Fourier optical imaging system to realize the imaging of translucent objects in turbid media solutions. The imaging method of translucent objects, which can realize the imaging of translucent objects, has the disadvantage that it cannot detect the movement process of a large number of particles in turbid solutions; the US patent "Method and device for characterization of particles in clear and turbid media" (authorization number is US7528384B2, the date of authorization is 2009.05.05), which can realize the detection of the size, shape and concentration of fluorescent particles, but the measurement accuracy is low, and real-time measurement cannot be realized, which limits its application scope; the patent "the change of particle size and concentration in turbid medium Dynamic Speckle Measurement Method" (publication number CN101788448A, publication date is 2010.07.28), this method can analyze the motion characteristics of particles through the change of particle size and concentration in turbid medium, and its shortcoming is that it does not consider the laser The non-uniformity of beam intensity distribution leads to low accuracy of measurement results

[0005] It can be seen from the analysis that in the measurement of the motion characteristics of the particles in the turbid medium, the main problems in the prior art are: the influence of the non-uniformity of the Gaussian laser beam intensity is not considered, and the dynamic, real-time and accurate measurement of the motion state of the particles in the turbid medium is not realized. detection

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