Meterhizium anisopliae and application thereof

A technology for Metarhizium anisopliae and Metarhizium anisopliae is applied in fungi, chemical recovery, microorganisms and other directions to achieve the effects of increasing yield, reducing production costs and stabilizing biological properties

Inactive Publication Date: 2012-08-08
丽江映华生物药业有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

Although mycelium grows fast at 30°C, it cannot form conidia well

Method used

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  • Meterhizium anisopliae and application thereof
  • Meterhizium anisopliae and application thereof
  • Meterhizium anisopliae and application thereof

Examples

Experimental program
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Effect test

Embodiment

[0049] ① Resurrection of the original species: at 27-30 0 Under the temperature condition of C, culture the preserved original species on the slant of the PDA medium test tube for 7-9 days, and prepare the bacterial suspension after the colonies cover the slant and piles of green conidia appear.

[0050] ② Preparation of bacterial suspension: Take 20ml of sterile water and add 70-80 glass beads (2-3mm in diameter) into a 250ml Erlenmeyer flask, and then transfer the cultured bacteria into the Erlenmeyer flask. Rotate the triangular flask on a shaker (200-260 rpm) for 0.5h, and dilute the bacterial suspension to 10 -5 After multiplying, spread on a PDA plate with a diameter of 150mm. Between 27 and 30 0 C for 7 days.

[0051] ③ Preparation of test tube slant: On the cultured PDA plate, select a single colony and inoculate it on the slant of a 10ml PDA test tube (20×200). 0 C for 7 days.

[0052] ④Preparation of primary seed liquid: Prepare 50ml of culture medium and place ...

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Abstract

The invention discloses a high-yield steroid fermentation meterhizium anisopliae novel strain LJYH201011 and application thereof to the preparation of mould oxide by using W-oxide as a substrate. The strain was collected in China General Microbiological Culture Collection Center (CGMCC) on Jan. 21st, 2011, and the collection number is CGMCC 4564. In the meterhizium anisopliae strain, the W-oxide is used as the substrate to produce the mould oxide when the meterhizium anisopliae strain is used in a conversion process of producing phytohormones by taking steroidal compounds (dioscin) as a raw material. The mould oxide produced by the meterhizium anisopliae strain has the characteristics of high conversion rate (70.86 to 78.74 percent), high yield (100 to 105 percent), relatively stable biological properties and the like. The invention can provide technical support for improving yield and lowering product cost in the preparation of plant steroidal hormones from dioscin serving as a raw material.

Description

technical field [0001] The invention belongs to the technical field of industrial fungi, and specifically designs a high-yield steroid fermentation Metarhizium anisopliae strain and an application technology for steroid compound fermentation. [0002] Background technique [0003] 1 Biological fermentation of steroidal compounds [0004] Steroids are a class of compounds containing cyclopentane polyhydrophenanthrene nuclei. Due to differences in substituents, double bond positions or stereo configurations on the mother nucleus, a series of compounds with unique physiological functions have been formed. Hormone drugs related to steroids are mainly: [0005] 1.1 Adrenocorticoids: referred to as corticosteroids, including mineralocorticoids and glucocorticoids. Mineralocorticoids mainly affect water and salt metabolism in the body, and the main drug is aldosterone, which is mainly used clinically to treat Addison's endocrine disease. Glucocorticoids mainly affect the metabol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P33/00C12R1/645
CPCY02P20/582
Inventor 周德群杨晓亮赵月华杨毅王钱钢
Owner 丽江映华生物药业有限公司
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