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New medicinal application of interleukin-12

A technology for new uses of interleukin, applied in drug combinations, extracellular fluid diseases, medical preparations containing active ingredients, etc., can solve problems such as death, infection, abnormality, etc., and achieve a wide range of applications and good results Effect

Inactive Publication Date: 2011-08-31
国科丹蓝生物科技(北京)有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the vast majority of patients with moderate to severe ARI will still experience serious complications such as bleeding and infection after passing through the acute phase, and eventually lead to death
The reason, on the one hand, is that most of the currently used hematopoietic growth factors can only promote the proliferation and differentiation of committed stem cells, but have little effect on pluripotent stem cells or progenitor cells. Although they can cause a short-term increase in peripheral blood cells, However, it lacks long-term effects; although it can make patients pass through the so-called "bone marrow nonfunctional period", it cannot achieve the reconstruction of hematopoietic function
On the other hand, if the patient's white blood cells are only elevated, the recovery of the damaged immune system and the reconstruction of immune function are not achieved, and the patient will still die due to low or abnormal immune function, leading to infections of various causes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Mouse Bone Marrow Mononuclear Cells (HSC) IL-12R Flow Cytometry Analysis

[0024] 1 Reagents and instruments

[0025] Reagents: RPMI-1640 (GIBCO, USA), fetal bovine serum (GIBCO, USA), Ficoll lymphocyte separation medium (Shanghai Huajing), CD3-PE, CD4-PreCP, CD8-FITC, IFN-γ-APC, Rat IgG1-APC and BFA were purchased from BD Pharmingen (Becton Dickinson / Phar Mingen MSA).

[0026] Instruments: flow cytometer (FACS Calibur, BD Company, the United States), SW-CJ-2F clean bench (Suzhou Antai Air Technology Co., Ltd.), carbon dioxide incubator (SANYO, Japan), LXJ-II centrifuge ( Shanghai Medical Analytical Instrument Factory), XW-80A Vortex Mixer (Shanghai Jingke Industrial Co., Ltd.).

[0027] 2 Experimental method:

[0028] (1) Preparation of bone marrow nucleated cells

[0029] Eight BALB / c mice were sacrificed by neck dislocation. The femurs on both sides were taken and put into the complete RPMI1640 culture medium supplemented with 10% fetal bovine serum. Th...

Embodiment 2

[0043] Example 2 Expression of HSC Apoptotic Rate in Mouse Bone Marrow Nucleated Cells After Radiation Irradiation

[0044] Purpose of the experiment: To observe the preventive and therapeutic effects of IL-12 on radiation-damaged cells

[0045] 1.1 Experimental method

[0046] SPF grade Kunming rats were selected and irradiated with 4Gy by a linear accelerator. The mice were administered once 24 hours before and 24 hours after radiation. They were killed 4 days after irradiation, and the apoptosis rate of bone marrow HSCs was detected. HSCs were labeled with c-kit APC and AnnexinVPE. It is an indicator of apoptosis detection.

[0047] (1) Preparation of bone marrow nucleated cells

[0048] BALB / c mice were sacrificed by neck dislocation, and bilateral femurs were taken under aseptic conditions, cultured with RPMI164 complete culture medium (with 10% fetal bovine serum added), and the bone marrow was obtained by repeatedly blowing and blowing with a syringe, and separated wi...

Embodiment 4

[0102] Example 4 The effect of rhIL-12 on the repair of radiation-damaged hematopoietic stem cells in neonatal umbilical cord blood

[0103] 1 Experimental materials

[0104] 1.1 Reagents

[0105] RPMI-1640 medium, Hank's solution, and fetal bovine serum were all products of GIBCO Company in the United States, Ficoll lymphocyte separation medium was purchased from Shanghai Huajing Biotechnology Company, Annexin V apoptosis signal detection kit, and anti-human CD34-FITC were purchased from BD Pharmingen Company (Becton Dickinson / Phar Mingen USA), RPMI-1640 complete culture solution is RPMI-1640 culture solution containing 10% fetal bovine serum.

[0106] 1.2 Instruments

[0107] Flow cytometer (FACS Calibur, BD company, the United States), SW-CJ-2F clean bench (Suzhou Antai Air Technology Co., Ltd.), carbon dioxide incubator (SANYO, Japan), LXJ-II centrifuge (Shanghai Medical Analytical Instrument Factory), XW-80A vortex mixer (Shanghai Jingke Industrial Co., Ltd.), linear a...

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Abstract

The invention relates to new medicinal application of interleukin-12. In the application, the interleukin-12 is applied to the preparation of medicaments for treating acute radiation sickness. Compared with the prior art, the invention has the advantages that: in the application of the interleukin-12 (interleukin-12, IL-12) to the preparation of the medicaments for treating the acute radiation sickness, the using range of the interleukin-12 is expanded, and new medicaments are provided for the treatment of the acute radiation sickness simultaneously; and results of pharmacodynamic experiments indicate that the interleukin-12 has a good effect on the acute radiation sickness.

Description

technical field [0001] The invention relates to a new drug application of interleukin-12 (interleukin-12, IL-12), especially the application of the drug prepared by its recombinant product produced by genetic engineering in the treatment of acute radiation sickness. Background technique [0002] With the widespread application of nuclear energy technology in the national economy, accidents in which people are exposed to large doses of radiation due to operational errors or violations of safety regulations also occur from time to time. After the Chernobyl nuclear power plant accident in the former Soviet Union in 1986, Brazil (1987), El Salvador (1989), Israel (1990), Belarus (1991), Russia (1997), Estonia (1994) and my country’s Shanghai (1990) and Jilin (1996) also had radiation accidents in which personnel were exposed. Therefore, the prevention and treatment of acute radiation sickness is a very important link in the emergency medical treatment of nuclear accidents, and i...

Claims

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Application Information

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IPC IPC(8): A61K38/20A61P7/06A61P17/16A61P37/02A61P39/00
Inventor 张宜俊夏书奇王翠玲王增松李文棠郭桂铃牛丽红罗群芳李平谢飞群宋阳
Owner 国科丹蓝生物科技(北京)有限公司
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