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Nitrogen responsive early nodulin gene

A specific, amino acid technology, applied in genetic engineering, climate change adaptation, sugar derivatives, etc., can solve problems such as lack of

Inactive Publication Date: 2011-08-24
UNIVERSITY OF GUELPH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although many nitrogen-responsive genes have been identified from the above studies, reports on how to use this knowledge to manipulate gene expression to make plants use nitrogen more efficiently have been lacking.

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0112] For the preparation of OsENOD93 overexpressing plants, introduction of polynucleotides into plant cells is accomplished by one of several techniques known in the art, including but not limited to electroporation or chemical transformation (see, e.g., Ausubel, ed. (1994) Current Protocols in Molecular Biology, John Wiley and Sons, Inc., Indianapolis, Indiana). Markers that confer resistance to toxic substances are used in the identification of transformed cells (cells that have taken up and expressed the test polynucleotide sequence) from non-transformed cells (those cells that do not contain or express the test polynucleotide sequence) useful in. In one aspect of the invention, genes are useful as markers for assessing the introduction of DNA into plant cells. A transgenic plant, transformed plant, or stably transformed plant, or any cell, tissue or seed of the foregoing, refers to a plant in which exogenous polynucleotides have been incorporated or integrated into pla...

Embodiment 1

[0171] [Example 1: Changes in biomass in plants exposed to different nitrogen levels]

[0172] Wild-type Japanese rice (Oryza sativa Japonica) var. Donjin plants were grown in a mixture of sphagnum moss and vermiculite (1:4) (SunGro Horticulture Canada Ltd. BC, Canada) with varying amounts of nitrate added once a week. Nutrient solution until harvest. Nutrient solution containing 4mM MgSO 4 , 5mM KCl, 5mM CaCl 2 , 1mMKH 2 PO 4 , 0.1mM Fe-EDTA, 0.5mM MES (pH6.0), 9μM MnSO 4 , 0.7 μM ZnSO 4 , 0.3 μM CuSO 4 , 46 μM NaB 4 o 7 and 0.2μM (NH 4 ) 6 Mo 7 o 2 . 10 mM nitrate was used as high nitrogen condition, 5 mM nitrate as medium nitrogen, and 1 mM nitrate as low nitrogen. Plants are grown under 16 hours light at 28~30℃(-400μmolm -2 the s -1 ) and 4 weeks in a growth room at 22-24°C for 8 hours in the dark. Shoots and roots were harvested independently, and differences in biomass were assessed as growth markers.

[0173] Plant growth measured by shoot biomass at m...

Embodiment 2

[0174] [Example 2: Response to nitrogen deficiency]

[0175] Wild-type Japanese rice (Oryza sativa Japonica) var. Donjin plants were grown as described in Example 1. Yellowing of leaves and presence of purple flavonoid anthocyanins are some typical responses of plants when N deficiency occurs (Diaz U, et al, Plant and Cell Physiology, 2006, 47:74-83). The relative anthocyanin content was analyzed based on the method described by Neff and Chory (Neff MM & Chory J, Plant Physiol, 1998, 118:27-35). Total chlorophyll was either measured using a Minolta SPAD 502DL chlorophyll meter (Tokyo, Japan), or extracted by ethanol and tested by a spectrophotometer according to Kirk (Kirk, JTO, Planta, Berl, 1968, 78:200). The levels of chlorophyll and anthocyanins in leaves were similar under high and medium nitrogen conditions. Under low nitrogen conditions, plants were not distinctly yellow or purple, although chlorophyll decreased and anthocyanins increased significantly compared to hig...

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Abstract

Isolated nucleic acids and proteins and plants expressing the same for improved nitrogen utilization, increased yield, and increased stress tolerance.

Description

[0001] 【CROSS-REFERENCE TO RELATED APPLICATIONS】 [0002] This application claims priority to US Provisional Patent Application No. 61 / 099,954, filed September 25, 2008, which is hereby incorporated in its entirety. 【Technical field】 [0003] The present invention generally relates to compositions and methods for modulating plant characteristics such as nitrogen use efficiency (NUE). 【Background technique】 [0004] Over the next 50 years, a combination of factors including increasing human populations, increasing crop use per capita, and many environmental concerns will place significant pressure on world crop production. Given current trends, it will be necessary to roughly double world productivity during this period, and a significant effort to meet the challenge of this economically and environmentally sustainable format (Rothstein SJ Plant Cell 2007, 19:2695-2699). Nitrogen is an essential element for plant growth and is considered to be the main control factor of plan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/82
CPCC07K14/415C07H21/04C12N15/8261C12N15/8251Y02A40/146
Inventor S·康德S·罗思坦Y-M·比J·克拉克
Owner UNIVERSITY OF GUELPH
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