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Method of double enzymatic coupling reaction for assisting extraction of solanesol

A technology for assisted extraction and solanesol, which is applied in the field of assisted extraction of solanesol by double-enzyme coupling method, can solve the problems of low yield and the like, and achieve the effect of less discharge of waste liquid

Inactive Publication Date: 2012-12-05
TIANSHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method uses cellulase to change the permeability of the potato leaf cell wall, and then uses lipase to hydrolyze the bound solanesol, which overcomes the traditional process of direct extraction followed by purification or reagent extraction followed by alkali saponification to cause solanesol degradation or isolysis Structured, resulting in a lower yield

Method used

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  • Method of double enzymatic coupling reaction for assisting extraction of solanesol

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of cellulase method of the present invention and lipase assist the method for extracting solanesol, comprise the steps:

[0023] 1) Crush the dried potato leaves (Longshu No. 6) and pass through a 60-mesh sieve. Accurately weigh 40 grams of potato leaves, add 800ml of distilled water, ultrasonically wash for 40 minutes under the conditions of ultrasonic power 150W, frequency 40KHz, and temperature 50°C, and then filter Obtain solid substance A;

[0024] 2) Add 600ml of cellulase solution prepared with pH 5.6 disodium hydrogen phosphate-citric acid buffer solution with a concentration of 0.002g / ml to substance A, and enzymolyze 5 at a temperature of 50°C and a stirring speed of 120r / min hours, and centrifuged at 4000 rpm for 13 min, then discarded the supernatant to obtain substance B;

[0025] 3) Add 200 ml of lipase solution with a concentration of 0.006 g / ml prepared with disodium hydrogen phosphate-potassium dihydrogen phosphate buffer solution of pH 8 to sub...

Embodiment 2

[0028] A method for extracting solanesol assisted by cellulase and lipase of the present invention comprises the following steps:

[0029] 1) Crush the dried potato leaves (Longshu No. 6) and pass through a 50-mesh sieve. Accurately weigh 40 grams of potato leaves, add 200ml of distilled water, ultrasonically wash for 60 minutes under the conditions of ultrasonic power 200W, frequency 40KHz, and temperature 30°C, and then filter Obtain solid substance A;

[0030] 2) Add 400ml of cellulase solution prepared with pH 4.0 disodium hydrogen phosphate-citric acid buffer solution with a concentration of 0.004g / ml to substance A, and enzymolyze 3 at a temperature of 30°C and a stirring speed of 110r / min. hours, and centrifuged at 3800 rpm for 10 min, then discarded the supernatant to obtain substance B;

[0031] 3) Add 600ml of lipase solution with a concentration of 0.003g / ml prepared with disodium hydrogen phosphate-potassium dihydrogen phosphate buffer solution of pH 9 to substanc...

Embodiment 3

[0034] A method for extracting solanesol assisted by cellulase and lipase of the present invention comprises the following steps:

[0035]1) Crush the dried potato leaves (Longshu No. 6) and pass through a 40-mesh sieve. Accurately weigh 40 grams of potato leaves, add 500ml of distilled water, ultrasonically wash for 20 minutes under the conditions of ultrasonic power 250W, frequency 40KHz, and temperature 60°C, and then filter Obtain solid substance A;

[0036] 2) Add 200ml of cellulase solution prepared with pH 4.8 disodium hydrogen phosphate-citric acid buffer solution with a concentration of 0.001g / ml to substance A, and enzymolyze 1 at a temperature of 60°C and a stirring speed of 100r / min hours, and centrifuged at 3600 rpm for 15 min, then discarded the supernatant to obtain substance B;

[0037] 3) Add 1000 ml of lipase solution prepared with disodium hydrogen phosphate-potassium dihydrogen phosphate buffer solution with a pH of 7 to a concentration of 0.001 g / ml into ...

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Abstract

The invention discloses a method of double enzymatic coupling reaction assisted extraction of solanesol. In the method, a cellulase is used for changing the permeability of cell walls of potato leaves and then lipase is used for hydrolyzing combined solanesol. The method overcomes the shortcoming of the lower yield caused by the degradation or isomerization of solanesol as the solanesol is extracted by the traditional process, then is purified or extracted with a reagent and finally is saponified with alkali.

Description

technical field [0001] The invention relates to a method for extracting solanesol by using dried potato leaves as raw materials and adopting a double-enzyme coupling method. Background technique [0002] Solanesol (Solanesol) is a long-chain semiterpene alcohol compound, which is an unsaturated nona-prenyl primary alcohol and belongs to tetrasesquiterpene alcohol. It has a unique chain-like isolated polyene structure and can be used as It is an important raw material for the synthesis of many drugs and plays a very important role in biochemistry. It mainly exists in Solanaceae plants, such as tobacco leaves, eggplant leaves, potato leaves, mulberry leaves, etc. Solanesol has the functions of antibacterial, anti-inflammatory, anti-ulcer and treatment of cardiovascular diseases. It is widely used in medicine and can be used as a synthetic raw material intermediate for ubiquinone drugs, antiallergic drugs, antiulcer drugs, hypolipidemic drugs and anticancer drugs. [0003] S...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/02
Inventor 张继王自社马君义杨玲娟王凤霞袁毅君谢天助
Owner TIANSHUI NORMAL UNIV
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