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Rhodosporidium paludigenum for degrading gossypol and application thereof

A technology of Rhodosporidium spore and free gossypol, applied in the field of microorganisms, can solve the problems of unstable performance of gossypol degrading bacteria, low feeding rate of cottonseed meal, shortage of protein feed resources, etc., so as to shorten the start-up time and achieve good removal effect. , The effect of low production and use cost

Inactive Publication Date: 2012-07-04
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a gossypol-degrading bacterial strain with high stability and strong gossypol-degrading ability aiming at the defect of unstable performance of gossypol-degrading bacteria in the prior art
The invention successfully solves the problems of low feeding rate of cottonseed meal and shortage of protein feed resources in the feed industry and breeding industry, and provides a basis for the wide and safe use of cottonseed meal

Method used

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  • Rhodosporidium paludigenum for degrading gossypol and application thereof
  • Rhodosporidium paludigenum for degrading gossypol and application thereof
  • Rhodosporidium paludigenum for degrading gossypol and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 strain mutagenesis and primary screening

[0020] 1) Rejuvenation of strains

[0021] Remove the liquid paraffin from the strain Rhodotorula HJ-Y obtained in the original test and stored at 4°C, put 1 mL of its bacterial suspension into a 250 mL Erlenmeyer flask with 100 mL of PDA liquid medium, and shake it in a constant temperature steam bath at 28°C. The bed was cultivated for 48 hours, and the rotation speed was 200r / min.

[0022] The screening method of the yeast HJ-Y is the same as that of Xia Xincheng, Li Guiqiang, Teng Anguo, etc. Screening and identification of strains for compound fermentation of cottonseed meal and optimization of fermentation process parameters, [J] Journal of Chinese Cereals and Oils, 2010, 25(1): 91-97 strain screening method.

[0023] 2) Strain selection

[0024] On the ultra-clean operating table, use the inoculation loop to pick up the rejuvenated bacterial liquid on the PDA solid medium plate for subculture, and select ...

Embodiment 2

[0041] Embodiment 2 Bacterial classification re-screening

[0042] The original species of HJ-D-20, HJ-D-30, HJ-Z-20-D-20 and the original species of control strain HJ-Y screened in Example 1 were respectively activated on the screening medium, and the activated The bacterial strains were inoculated on the slant of the test tube (see the screening medium for the formula) for later use. The test tube species were respectively inoculated in 1000ml shake flasks containing 200ml potato liquid medium, cultured with constant temperature shaking until the logarithmic phase, and the activated strains were inserted into 20ml potato liquid culture to obtain the first-grade strains based on the 100ml Erlenmeyer flask (the condition was 30 ℃, 200r / min shaker culture for 48h), take the first-grade strain and insert 1ml into a 250ml Erlenmeyer flask equipped with 100ml potato liquid medium as the second-grade strain (conditions are 30°C, 200r / min shaker culture for 48h ).

[0043] Use HJ-...

Embodiment 3

[0048] 1) Inject Rhodosporidium yeast HJ-D-20 (CGMCC No.4372) into 20ml potato liquid culture to obtain the first-grade strain based on a 100ml Erlenmeyer flask (conditions are 30°C, 200r / min shaker culture for 48h), take one Inject 1 ml of the first-grade strain into a 250-ml Erlenmeyer flask equipped with 100 ml of potato liquid medium to be the second-grade strain (conditions are 30° C., 200 r / min shaker culture for 48 h).

[0049] 2) The secondary bacterial strains cultivated to the logarithmic phase were respectively inserted into the cottonseed meal fermentation substrate with a free gossypol content of 614mg / kg, the inoculum size was 5%, placed in a 500ml Erlenmeyer flask, 30°C, constant temperature and static Place and culture for 72h. Dry, measure FG and CP content, analyze fermentation effect, see Table 3. It can be seen from Table 3 that after fermentation by strain HJ-D-20, the degradation rate of FG reached 90.80%, the increase rate of crude protein was 24.59%, a...

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Abstract

The invention discloses Rhodosporidium paludigenum for degrading gossypol and application thereof, belonging to the field of microorganisms. A strain of the Rhodosporidium paludigenum HJ-D-20 is preserved in China General Microbiological Culture Collection Center on Nov. 26th, 2010 and has a culture preservation number of CGMCC No.4372. The strain has the degradation rate of 90.78% to gossypol acetate and the CP (Crude Protein) enhancement rate of 24.68%, has favorable stability, and ensures cotton seed meal to reach a use standard so as to increase the utilization rate of the cotton seed meal. The Rhodosporidium paludigenum disclosed by the invention has great importance for alleviating the shortage in protein resources, reducing soybean import and cotton seed meal export to Japan at lowprice, controlling the continuous increase of the price of the protein resources and reinforcing nation power.

Description

technical field [0001] The invention belongs to the field of microbes, and relates to a rhodosporidium yeast for degrading gossypol and application thereof. technical background [0002] Cottonseed meal is an important plant protein feed resource, which can make up for the shortage of protein resources to a certain extent, but due to the existence of gossypol, low and unbalanced amino acid content and high crude fiber content in cottonseed meal, its application in the aquaculture industry is limited. A large number of widely used. A certain amount of free gossypol (FG) in the diet will have negative effects on animal growth, reproduction, gastrointestinal tract and body tissues and organs. The use of microorganisms to ferment cottonseed meal can not only greatly reduce the FG content and play a role in detoxification, but also can significantly increase the nutritional value of cottonseed meal, so that its use in livestock and poultry feed can be significantly increased. S...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16A23L1/015A62D3/02C12R1/645A62D101/28A23L5/20
Inventor 高峰林海晶夏新成江芸周光宏
Owner NANJING AGRICULTURAL UNIVERSITY
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