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Gossypol acetate degrading bacterium S6

A technology of gossypol acetate and degrading bacteria is applied in the field of biological high, which can solve the problems of lack of theoretical basis for process conditions, unclear synergy or resistance mechanism of compound fermentation strains, etc., so as to shorten the start-up time, reduce the cheap export to Japan, and achieve good stability. sexual effect

Inactive Publication Date: 2011-08-17
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

In recent years, the research on fermented cottonseed meal has mainly focused on the selection of virus-free strains and the research on the effect of virus-free strains, but the mechanism of synergistic or antagonistic effects between the compound fermentation strains is unclear, and the process conditions still lack sufficient theoretical basis

Method used

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  • Gossypol acetate degrading bacterium S6
  • Gossypol acetate degrading bacterium S6

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Embodiment Construction

[0018] Examples of the present invention are described below.

[0019] The original species of several strains screened in this experiment were activated on the screening medium, and the degradation performance was measured, and they were inoculated on the slant of the test tube for later use. The test tube seed was inoculated in a 1000ml shake flask containing 200ml potato liquid medium, and cultured with constant temperature shaking until the logarithmic phase, and the activated strain was inserted into 20ml potato liquid culture to obtain a first-class strain based on a 100ml Erlenmeyer flask (conditions were 30°C, 200r / min shaker culture 48h), take the primary strain and inoculate 1ml into a 250ml Erlenmeyer flask equipped with 100ml potato liquid medium as the secondary strain (conditions are 30°C, 200r / min shaker culture 48h). The formulation of gossypol acetate sugar-free Chapei medium is: gossypol acetate 3g / L, NaNO3 2g / L, KCl 0.5g / L, FeSO4 0.01g / L, MgSO4 7H2O 0.5g / L, ...

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Abstract

The invention provides a gossypol acetate degrading bacterium S6 capable of degrading free gossypol. The identification result shows that the gossypol acetate degrading bacterium S6 belongs to Saccharomycetes, Saccharomycetaceae, Kloeckeralindneri. The main biological characteristics are as follows: the gossypol acetate degrading bacterium S6 is Kloeckera, is milk white in color and is round in shape; the gossypol acetate degrading bacterium S6 grows in a potato agar culture medium, and can grow in a Czapeks medium for glucose fermentation; and the gossypol acetate degrading bacterium S6 can grow by using 0.3% gossypol acetate as the unique carbon source. Besides, the strain has favorable stability, thereby ensuring that the cotton seed pulp meets use standards and improving the utilization ratio of the cotton seed pulp. The gossypol acetate degrading bacterium S6 provided by the invention is of great importance in alleviating protein resource constraints, reducing soybean import and low-cost cotton seed pulp export to Japan, controlling the continuously rising price of protein resources and enhancing the national strength.

Description

1. Technical field [0001] The invention relates to a high-concentration gossypol-tolerant bacterial strain, which belongs to the high-tech field of biology, uses microorganisms to degrade free gossypol, and is suitable for the biological treatment of modern cottonseed meal with high-concentration gossypol content. 2. Technical background [0002] Cottonseed meal is an important plant protein feed resource, but due to the existence of gossypol, low and unbalanced amino acid content and high crude fiber content in cottonseed meal, it has become the main factor limiting its wide use in aquaculture. A certain amount of gossypol (FG) in the diet can have negative effects on animal growth, reproduction, gastrointestinal tract and body tissues and organs. "Sealing" makes FG lose its toxicity, and it is difficult to be digested and absorbed by animals after it is combined. However, it has been reported that the combination of gossypol can release FG during animal digestion, resultin...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12R1/645
Inventor 高峰夏新成林海晶江芸周光宏
Owner NANJING AGRICULTURAL UNIVERSITY
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