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Gossypol degrading strain and application thereof

A technology of strain and free gossypol, applied in the field of microorganisms, can solve the problems of shortage of protein feed resources, unstable performance of gossypol-degrading bacteria, low feed rate of cottonseed meal, etc., and achieves shortened start-up time, good removal effect, and production use. low cost effect

Inactive Publication Date: 2012-10-03
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a gossypol-degrading bacterial strain with high stability and strong gossypol-degrading ability aiming at the defect of unstable performance of gossypol-degrading bacteria in the prior art
The invention successfully solves the problems of low feeding rate of cottonseed meal and shortage of protein feed resources in the feed industry and breeding industry, and provides a basis for the wide and safe use of cottonseed meal

Method used

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  • Gossypol degrading strain and application thereof
  • Gossypol degrading strain and application thereof
  • Gossypol degrading strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 strain mutagenesis and primary screening

[0020] 1) Rejuvenation of strains

[0021] Remove the liquid paraffin from the bacterial strain GJ-Y stored at 4°C obtained in the original test, put 1mL of its bacterial suspension into a 250mL Erlenmeyer flask with 100mL of PDA liquid medium, and cultivate it on a constant temperature steam bath shaker at 28°C 48h, the speed is 200r / min.

[0022] 2) Strain selection

[0023] On the ultra-clean operating table, use the inoculation loop to pick up the rejuvenated bacterial liquid on the PDA solid medium plate for subculture, and select the pure strain that grows well, has stable properties and grows as a single colony. The purified single strains were transferred to the Chase culture medium plate, and after 5 passages, the preserved strains were used for mutagenesis.

[0024] 3) Pre-cultivation of the mutagenesis starting strain

[0025] Inoculate the selected starting strains for mutagenesis on 0.3% gossypol-s...

Embodiment 2

[0040] Embodiment 2 Bacterial classification re-screening

[0041] The original species of GJ-D-25 and GJ-D-20 bacterial strains screened in Example 1 and the original species of control strain GJ-Y were activated on the screening medium respectively, and the activated bacterial classifications were inoculated on the test tube slant (formula See Screening Medium) for later use. The test tube seed was inoculated in a 1000ml shake flask containing 200ml potato liquid medium, cultured with constant temperature shaking until the logarithmic phase, and the activated strain was inserted into 20ml potato liquid culture to obtain a first-class strain based on a 100ml Erlenmeyer flask (the condition was 30°C , 200r / min shaker culture for 48h), take the first-grade strain and insert 1ml into a 250ml Erlenmeyer flask equipped with 100ml potato liquid medium as the second-grade strain (conditions are 30°C, 200r / min shaker culture for 48h) . The screening medium formula is: gossypol acet...

Embodiment 3

[0047] 1) Inoculate Candida yeast GJ-D-25 (CGMCC No.4373) into 20ml potato liquid culture to obtain first-grade strains based on a 100ml Erlenmeyer flask (conditions are 30°C, 200r / min shaker culture for 48h), take the first-grade Inject 1 ml of the strain into a 250 ml Erlenmeyer flask containing 100 ml of potato liquid medium as the secondary strain (conditions are 30° C., 200 r / min shaker culture for 48 h).

[0048] 2) Secondary strains cultivated to the logarithmic phase were respectively inoculated into 100 g of cottonseed meal substrate with an inoculum size of 5%, placed in a 500 ml Erlenmeyer flask, and incubated at a constant temperature at 30° C. for 72 hours. Dry, measure FG and CP content, and analyze the fermentation effect.

[0049] The analysis of the fermentation effect is shown in Table 3. After fermentation by the strain GJ-D-25, the degradation rate of FG reached 91.40%, the increase rate of crude protein was 24.81%, and the degradation rate of gossypol was ...

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Abstract

The invention belongs to the field of microorganisms and discloses a gossypol degrading strain and application thereof. The strain of candida railenensis GJ-D-25 (Candidarailenensis) is preserved in the China general microbiological culture collection center (CGMCC) on Nov. 26th in 2010. The strain has a preservation number of CGMCC No.4373. The degrading rate of the strain to gossypol acetic acid is 91.40 percent. A CP (Crude Protein) improvement rate is 24.99 percent. The stain has high stability and ensures that cotton seed meal reaches the using standard so as to improve the utilizing rate of the cotton seed meal. The gossypol degrading strain has important significances for relieving shortage of protein resources, reducing imported soybeans and low-price export of the cotton seed meal to the Japan, controlling continuous rise in price of the protein resources and enhancing national strength.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a gossypol-degrading bacterial strain and application thereof. technical background [0002] Cottonseed meal is an important plant protein feed resource, which can make up for the shortage of protein resources to a certain extent, but due to the existence of gossypol, low and unbalanced amino acid content and high crude fiber content in cottonseed meal, its application in the aquaculture industry is limited. A large number of widely used. A certain amount of free gossypol (FG) in the diet will have negative effects on animal growth, reproduction, gastrointestinal tract and body tissues and organs. The use of microorganisms to ferment cottonseed meal can not only greatly reduce the FG content and play a role in detoxification, but also can significantly increase the nutritional value of cottonseed meal, so that its use in livestock and poultry feed can be significantly increased. Stu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16A23L1/015C12R1/72A23L5/20
Inventor 高峰林海晶夏新成江芸周光宏
Owner NANJING AGRICULTURAL UNIVERSITY
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