Method for preparing stevia rebaudiana biological growth promoting agent
A growth agent, stevia technology, applied in the field of preparation of biological growth agents, can solve the problems of waste of resources, environment, leaf waste residue cannot be effectively used, pollution, etc.
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Embodiment 1
[0015] Raw materials: 10kg of stevia leaf waste residue, 15kg of stems, quicklime, microbial composite strains (composed of nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium-solving bacteria, photosynthetic bacteria, actinomycetes, microorganisms producing agricultural antibiotics, yeast, bacillus, Bifidobacteria, lactic acid bacteria, Clostridium butyricum and other beneficial microorganisms, the number of beneficial viable bacteria is more than 2 billion / g), inorganic salts (sodium selenite 26.3g, germanium dioxide 4.3g, zinc sulfate heptahydrate 13.2g, borax 52.0g), urea 1kg, made into 30kg;
[0016] step:
[0017] 1) Take the leaf waste residue and stem stems extracted from Stevia rebaudiana and grind them separately, the particle size is 30-50 mesh, mix the powder of leaves and stems, and maintain the moisture content. Put the raw materials into a cooking tank, add water and cook for 2 hours at a temperature of 70°C, and the liquid enters the storage ...
Embodiment 2
[0021] Determination of total solids of stevia biological growth agent
[0022] 1) Suspension and supernatant adopt ISO 2173-78 "Refractometer method for the determination of soluble solids in fruits and vegetables and their products". Mix the sample thoroughly, squeeze out the filtrate with four layers of gauze, discard the first few drops, and collect the filtrate.
[0023] 2) Dip 2 to 3 drops of the test solution with a glass rod, drop it on the center of the prism surface of the refractometer, quickly close the prism, and let it stand for 1 min, so that the test solution is uniform without bubbles and fills the field of vision. Align the light source and observe the objective lens through the eyepiece. Adjust the indicator gauge to divide the field of view into light and dark parts, and then rotate the fine-tuning screw to make the boundary between light and dark clear, and make the dividing line just on the cross point of the objective lens. Read the percent or refracti...
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